产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类beta肌动蛋白抗体
目录 :
ATGA0457
规格 :
100微升
价格 :
290美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT4G6
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
NKMAX 产品网页
产品信息
产品类型 :
抗体
产品名 :
人类beta肌动蛋白抗体
目录号(SKU号) :
ATGA0457
NCBI :
NP_001092
描述 :
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
别称 :
Actin, cytoplasmic 1, Beta-actin
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Gunning PW., et al. (2015) Journal of Cell Science. 128(11): 2009-2019.;;Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163(4): 673-8.
ab宿主 :
小鼠
抗体亚型 :
IgG1 kappa
免疫原 :
重组人类ACTB(1-375aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0457 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: HeLa cell lysate ;;Lane 3.: Jurkat cell lysate;;Lane 4.: PC3 cell lysate;;Lane 5.: A431 cell lysate;;Lane 6.: HepG2 cell lysate;;Lane 7.: MCF7 cell lysate;;Lane 8.: U87-MG cell lysate;;Lane 9.: NIH-3T3 cell lysate The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Brain tissue lysate;;Lane 2.: Kidney tissue lysate;;Lane 3.: Liver tissue lysate;;Lane 4.: Lung tissue lysate;;Lane 5.: Spleen tissue lysate;;Lane 6.: Testis tissue lysate;;Lane 7.: Bone marrow tissue lysate;;Lane 8.: Stomach tissue lysate {ATGA0457-WB2.jpg} The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: ACTB antibody 1:1000;;Lane 2.: ACTB antibody 1:3000;;Lane 3.: ACTB antibody 1:5000;;Lane 4.: ACTB antibody 1:10000 {ATGA0457-WB3.jpg}
反应对象 :
人类
克隆编号 :
AT4G6
Ab用途 :
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
抗原物种 :
人类
基因类别 :
细胞骨架
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P60709
内部搜索条款 :
b肌动蛋白, beta 肌动蛋白
规格1 :
100微升
价格1(美元) :
290美元
更多信息或购买 :
NKMAX 产品网页
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
salesbio@atgenglobal.com
http://www.atgenglobal.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。