抗体

人类Akt1抗体

ATGA0480

50微升

170美元

单克隆

小鼠

未共轭

AT6D5

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类Akt1抗体

ATGA0480

NP_001014432

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

RAC甲丝氨酸/苏氨酸蛋白激酶, AKT, CWS6, PKB, PKB-甲, PRKBA, RAC, RAC-甲

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lindhurst MJ, et al (2011) N Engl J Med. 365(7): 611-9.

小鼠

IgG2a kappa

重组人类AKT1(1-480aa)纯化来自昆虫细胞

酶联免疫吸附测定, 免疫印迹, 流式细胞仪, 免疫细胞化学/IF

ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0480-IF1b.jpg}

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: NIH3T3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Recombinant Human AKT1 ;;Lane 2.: Recombinant Human AKT2 ;;Lane 3.: Recombinant Human AKT3 {ATGA0480-WB2.jpg}

人类

AT6D5

The antibody has been tested by Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

人类

信号转导

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P31749

Akt1, RAC甲丝氨酸/苏氨酸蛋白激酶, AKT, CWS6, PKB, PKB-甲, PRKBA, RAC, RAC-甲, ATGA0480-10微克, ATGA0480-20微克, ATGA0480-50微克, ATGA0480-100微克, ATGA0480-250微克, ATGA0480-500微克, ATGA0480-1毫克, ATGA0480-10, ATGA0480-20, ATGA0480-50, ATGA0480-100, ATGA0480-250, ATGA0480-500, ATGA0480-1