抗体

人类PEDF/SERPINF1抗体

ATGA0484

50微升

170美元

单克隆

小鼠

未共轭

AT13D9

免疫印迹, 酶联免疫吸附测定, 流式细胞仪

人类PEDF/SERPINF1抗体

ATGA0484

NP_002606.3

Pigment epithelium-derived factor (PEDF) is a 50kDa glycoprotein and member of the serpin superfamily without any activity of serine protease. PEDF is an angiogenesis inhibitor with neurotrophic properties, balances angiogenesis in the eye and blocks tumor progression. That is, in neuronal cell, PEDF is promoting cell survival and differentiation, however, when endothelial cells are exposed to PEDF, their cell migration and proliferation are inhibited, and they undergo apoptosis.

SERPINF1, Serpin peptidase inhibitor clade F member 1, Proliferation inducing protein 35, Pigment epithelium-derived factor EPC 1, Pigment epithelium-derived factor, PIG35, PI, GSTP1, GST3, Glutathione S-Transferase Pi 1, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S-transferase P, Glutathione S Transferase Pi, FAEES3, EPC1, DFN7, APF0619

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Baba H. et al (2005) Arteriosler Thromb Vasc Biol. 25: 1938-1944.;; Filleur S. et al (2005) Cancer Res. 65: 5144-5152.;; Hattenbach LO. et al (2004) Ophthalmic Res. 37: 341-346.

小鼠

IgG1 kappa

重组人类PEDF(20-418aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 流式细胞仪

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: PEDF transfected 293T cell lysate

人类

AT13D9

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of PEDF in K562 cells. The cell was stained with ATGA0484 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

人类

激素&细胞因子

APF0619 has been replaced with a catalog number ATGA0484.

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P36955

EPC1, EPC-1, Glutathione S-Transferase pi1, GSTP1, IL 1 A, IL 1 alpha, IL 1A, IL1 alpha, IL-1 alpha, IL1A, IL-1A, IL1alpha, IL1F1, Interleukin 1 alpha, Interleukin 1-alpha, Interleukin-1 alpha, Interleukin-1 alpha precursor, PEDF, PIG 35, PIG35, Pigment epithelium derived factor, Pigment epithelium derived factor EPC 1, Pigment epithelium-derived factor, Pigment epithelium-derived factor EPC 1, Proliferation inducing protein 35, Serpin F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, SERPINF1, SERPIN-F1, ATGA0484-10ug, ATGA0484-20ug, ATGA0484-50ug, ATGA0484-100ug, ATGA0484-250ug, ATGA0484-500ug, ATGA0484-1mg, ATGA0484-010, ATGA0484-020, ATGA0484-050, ATGA0484-100, ATGA0484-250, ATGA0484-500, ATGA0484-01M