产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类ATG3抗体
目录 :
ATGA0493
规格 :
50微升
价格 :
170美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT15F6
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类ATG3抗体
目录号(SKU号) :
ATGA0493
NCBI :
NP_071933
描述 :
Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy.
别称 :
ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Tanida I., et al. (2002) J Biol Chem. 277: 13739-13744. ;; Yamada Y., et al. (2007) J Biol Chem. 282: 8036-8043.
ab宿主 :
小鼠
抗体亚型 :
IgG1 kappa
免疫原 :
重组人类ATG3(1-314aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 流式细胞仪, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of ATG3 in HeLa cells. The cell was stained with ATGA0493 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). {ATGA0493-IF1b.jpg}
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: 293T cell lysate ;;Lane 2.: ATG3 transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: K562 cell lysate ;;Lane 2.: Raji cell lysate ;;Lane 3.: Jurkat cell lysate {ATGA0493-WB2.jpg}
反应对象 :
人类
克隆编号 :
AT15F6
Ab用途 :
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of ATG3 in HeLa cells. The cell was stained with ATGA0493 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
抗原物种 :
人类
基因类别 :
Ubiquitination
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
Q9NT62
内部搜索条款 :
ATG3, ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96,ATGA0493-10ug,ATGA0493-20ug,ATGA0493-50ug,ATGA0493-100ug,ATGA0493-250ug,ATGA0493-500ug,ATGA0493-1mg,ATGA0493-10,ATGA0493-20,ATGA0493-50,ATGA0493-100,ATGA0493-250,ATGA0493-500,ATGA0493-1
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司，制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。