免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP). BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.
BCL2-antagonist/killer 1, Apoptosis regulator BAK, BAK, BAK-LIKE, Bcl-2 homologous antagonist/killer, Bcl2-L-7, BAK-1, BAK 1, BAK like, Bak NT, BAK1, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl2 homologous antagonist killer, Bcl2 like 7 Protein, BCL2-antagonist/killer 1, BCL2L7, CDN 1, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK
1mg/ml (determined by BCA assay)
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Birgit Kemmerling, et al. (2008) Plant Signal Behav, 3(2): 116-118.;;Libo Shan, et al. (2008) Cell Host Microbe, 4(1): 17-27.;;Xu-Bao Shi, et al. (2007) Proc Natl Acad Sci U S A, 104(50): 19983-19988.
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of BAK in HeLa cells. The cell was stained with ATGA0523 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1 : 293T cell lysate;;Lane 2 : HeLa cell lysate;;Lane 3 : A431 cell lysate;;Lane 4 : A549 cell lysate;;Lane 5 : Jurkat cell lysate ;;Lane 6 : MCF7 cell lysate ;;Lane 7 : PC3 cell lysate
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ATGA0523 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Additional Comment Only :
This product was produced from tissue culture supernatant.
Clonality Mono VS Poly :
UniProt No. :
BAK, ATGA0523-10微克, ATGA0523-20微克, ATGA0523-50微克, ATGA0523-100微克, ATGA0523-250微克, ATGA0523-500微克, ATGA0523-1毫克, ATGA0523-10, ATGA0523-20, ATGA0523-50, ATGA0523-100, ATGA0523-250, ATGA0523-500, ATGA0523-1
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