抗体

人类RASSF1A抗体

ATGA0528

50微升

190美元

单克隆

小鼠

未共轭

AT3F3

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类RASSF1A抗体

ATGA0528

NP_009113

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.

RASSF1(Ras association (RalGDS/AF-6) domain family 1),123F2, RDA32, NORE2A, RASSF1A, REH3P21, Ras association (RalGDS/AF-6) domain family member 1

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Agathanggelou A, et al., (2005) Cancer Res. 65, 3497-3508.;; Lerman MI. et. al. (2000) Cancer Res. 60, 6116-6133. ;;Strunnikova M. et al., (2005) Mol Cell Biol. 25, 3923-3933. ;;Dammann R. et al., (2005) Histol Histopathol. 20, 645-663. ;;Rossa W.k, et al., (2007) American Journal of pathology. 170, 941-950.

小鼠

IgG1 kappa

重组人类RASSF1A(1-340aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of RASSF1A in HeLa cells. The cell was stained with ATGA0528 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: RASSF1A Transfected 293T cell lysate{2004010467743607.jpg}

人类

AT3F3

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

信号转导

This product was produced from tissue culture supernatant.

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

Q9NS23

RASSF1A, ATGA0528-10微克, ATGA0528-20微克, ATGA0528-50微克, ATGA0528-100微克, ATGA0528-250微克, ATGA0528-500微克, ATGA0528-1毫克, ATGA0528-10, ATGA0528-20, ATGA0528-50, ATGA0528-100, ATGA0528-250, ATGA0528-500, ATGA0528-1

Li W, et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. (Cell Death Differ. 2019){https://pubmed.ncbi.nlm.nih.gov/30315205/};;Guo M, et al. RASSF1-AS1, an antisense lncRNA of RASSF1A, inhibits the translation of RASSF1A to exacerbate cardiac fibrosis in mice. (Cell Biol Int. 2019){https://pubmed.ncbi.nlm.nih.gov/30571844/};;Dabral S, et al. A RASSF1A-HIF1α loop drives Warburg effect in cancer and pulmonary hypertension. (Nat Commun. 2019){https://pubmed.ncbi.nlm.nih.gov/31086178/};;Francisco J, et al. The tumor suppressor RASSF1A modulates inflammation and injury in the reperfused murine myocardium. (J Biol Chem. 2019){https://pubmed.ncbi.nlm.nih.gov/31311858/};;Chen J, et al. 5-Aza-CdR Regulates RASSF1A By Inhibiting DNMT1 To Affect Colon Cancer Cell Proliferation, Migration And Apoptosis. Cancer Manag Res. 2019{https://pubmed.ncbi.nlm.nih.gov/31807076/};;Slemmons KK, et al. Targeting Hippo-Dependent and Hippo-Independent YAP1 Signaling for the Treatment of Childhood Rhabdomyosarcoma. (Cancer Res. 2020){https://pubmed.ncbi.nlm.nih.gov/32354737/};;Dhanaraman T, et al. RASSF effectors couple diverse RAS subfamily GTPases to the Hippo pathway. Sci Signal. 2020{https://pubmed.ncbi.nlm.nih.gov/33051258/};;Jeon HJ, Oh JS. RASSF1A Regulates Spindle Organization by Modulating Tubulin Acetylation via SIRT2 and HDAC6 in Mouse Oocytes. (Front Cell Dev Biol. 2020){https://pubmed.ncbi.nlm.nih.gov/33195286/};;Jia J, et al. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein. (J Cell Mol Med. 2021){https://pubmed.ncbi.nlm.nih.gov/33960627/};;Roßwag S, et al. RASSF1A-Mediated Suppression of Estrogen Receptor Alpha (ERα)-Driven Breast Cancer Cell Growth Depends on the Hippo-Kinases LATS1 and 2. (Cells. 2021){https://pubmed.ncbi.nlm.nih.gov/34831091/}