抗体

人类PP2C甲/PPM1A抗体

ATGA0529

50微升

190美元

单克隆

小鼠

未共轭

p6c7

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类PP2C甲/PPM1A抗体

ATGA0529

NP_066283

Protein phosphatase 2C (PP2Calpha) is a Mn2+ - or Mg 2+ -dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNk MAPk pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signalingkinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2Calpha, the prototypical member of the PPM family.

Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2CA, PP2C alpha, PDPC 1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lin X, et al., (2006) Cell. 125(5): 915-928. ;;Duan X, et al., (2006) J Biol Chem. 281(48):36526-32.

小鼠

IgG2b kappa

重组人类PP2Calpha/PPM1A(1-382aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained with ATGA0529 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha/PPM1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1 : Jurkat cell lysate;;Lane 2 : HeLa cell lysate;;Lane 3 : K-562 cell lysate;;Lane 4 : MCF7 cell lysate;;Lane 5 : A549 cell lysate;;Lane 6 : Raji cell lysate;;Lane 7 : Mouse kidney tissue lysate;; Lane 8 : Mouse brain tissue lysate;;Lane 9 : Mouse liver tissue lysate

人类

p6C7

The antibody has been tested by ELISA , Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

Cancer

This product was produced from tissue culture supernatant.

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P35813

Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2C-alpha, PP2Calpha, PP2CA, PP2C A, PP2C alpha, PDPC 1, PDP1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43, ATGA0529-10ug, ATGA0529-20ug, ATGA0529-50ug, ATGA0529-100ug, ATGA0529-250ug, ATGA0529-500ug, ATGA0529-1mg, ATGA0529-010, ATGA0529-020, ATGA0529-050, ATGA0529-100, ATGA0529-250, ATGA0529-500, ATGA0529-01M

Kim JS, et al. The interaction of hepatitis B virus X protein and protein phosphatase type 2 Calpha and its effect on IL-6. (Biochem Biophys Res Commun. 2006){https://pubmed.ncbi.nlm.nih.gov/17055456/};;Wang K, et al. TGF-β1/p65/MAT2A pathway regulates liver fibrogenesis via intracellular SAM. (EBioMedicine. 2019){https://pubmed.ncbi.nlm.nih.gov/30926424/};;Wang Z, et al. MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation. (Allergy Asthma Immunol Res. 2020){https://pubmed.ncbi.nlm.nih.gov/32638565/};;Yang J, et al. Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine. (Exp Ther Med. 2020){https://pubmed.ncbi.nlm.nih.gov/32742335/};;Xia YC, et al. lncRNA TSPEAR-AS2, a Novel Prognostic Biomarker, Promotes Oral Squamous Cell Carcinoma Progression by Upregulating PPM1A via Sponging miR-487a-3p. (Dis Markers. 2021){https://pubmed.ncbi.nlm.nih.gov/34336002/};;Feng D,et al. Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage. (Sci Adv. 2022){https://pubmed.ncbi.nlm.nih.gov/36179025/}