抗体

人类VHL抗体

ATGA0542

50微升

190美元

单克隆

小鼠

未共轭

AT82B10

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类VHL抗体

ATGA0542

NP_000542

Von Hippel-Lindau disease (VHL) is a dominant inherited syndrome characterized by the predisposition todevelop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas andhemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in theVHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-typeallele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich in beta sheet (beta-domain) anda smaller alpha-helical domain (alpha-domain), held together by two linkers and a polar interface. VHL proteinis also involved in the degradation of hypoxia-inducible factor (HIF).

Von Hippel-Lindau tumor suppressor isoform 1, Von Hippel-Lindau tumor suppressor isoform 1,HRCA1, RCA1, VHL1, Von Hippel-Lindau tumor suppressor isoform 1 pVHL, G7 protein, Elongin bindingprotein, HRCA 1, RCA 1, VHL, VHL 1, VHLH, Von Hippel Lindau disease tumor suppressor, vonHippel Lindau syndrome, von Hippel Lindau tumor suppressor, AVH0616

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Latif F., et al. (1993) Science. 260(5112): 1317-20.;;Duan DR., et al. (1995) PNAS. 92(14): 6459-63.;;Maxwell PH., et al. (1999) Nature. 399(6733): 271-5.;;

小鼠

IgG2b kappa

重组人类VHL(1-154aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of VHL in Hep3B cells. The cell was stained with ATGA0542 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: HeLa cell lysate;; Lane 3.: Raji cell lysate;;Lane 4.: Jurkat cell lysate;;Lane 5.: A549 cell lysate;;Lane 6.: MCF7 cell lysate;;Lane 7.: PC3 cell lysate;;

人类

AT82B10

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

Cancer

This product was produced from tissue culture supernatant.

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P40337

VHL, ATGA0542-10微克, ATGA0542-20微克, ATGA0542-50微克, ATGA0542-100微克, ATGA0542-250微克, ATGA0542-500微克, ATGA0542-1毫克, ATGA0542-10, ATGA0542-20, ATGA0542-50, ATGA0542-100, ATGA0542-250, ATGA0542-500, ATGA0542-1