抗体

人类ISG15/UCRP抗体

ATGA0543

50微升

190美元

单克隆

小鼠

未共轭

300000

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类ISG15/UCRP抗体

ATGA0543

NP_005092

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play importantroles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety ofproteins when cells are treated with type I interferon or lipopolysaccharide.

Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP,IFI15,ISG15,Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene,15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDaprotein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, InterferonStimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier,ubiquitin cross reactive protein precursor

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jonathan D et al.,(1996) Proc Natl Acad Sci USA 93:211-215.;;Weiming Y et al.,(2001) EMBO J 20(3):362-371.;;Chen Z et al.,(2005) Proc Natl Acad Sci USA 102(29):10200-10205.;;

小鼠

IgG1 kappa

重组人类ISG15(1-157aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of ISG15 in HeLa cells. The cell was stained with ATGA0543 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: MCF7 cell lysate;;Lane 2.: PC3 cell lysate;;

人类

300000

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

Epigenetics and Nuclear Signaling

This product was produced from tissue culture supernatant.

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P05161

ISG15, UCRP, ATGA0543-10微克, ATGA0543-20微克, ATGA0543-50微克, ATGA0543-100微克, ATGA0543-250微克, ATGA0543-500微克, ATGA0543-1毫克, ATGA0543-10, ATGA0543-20, ATGA0543-50, ATGA0543-100, ATGA0543-250, ATGA0543-500, ATGA0543-1