抗体

人类亲环素类B/PPIB抗体

ATGA0545

50微升

190美元

单克隆

小鼠

未共轭

k2E2

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

人类亲环素类B/PPIB抗体

ATGA0545

NP_000933

Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin.

Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase B, Cphn-2, Cyclophilin B, CYP-20b, CYPB, CYP-S1, OI9, Rotamase B, S-cyclophilin, SCYLP

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Watashi K, et al., (2005) Mol Cell 19(1):111-112.;;Yurchenko V, et al., (2001) Biochem Biophys Res Commun 288(4):786-788.;;E.Roydon P, et al., (1991) Proc Natl Acad Sci USA 88:1903-1907.

小鼠

IgG1 kappa

重组人类亲环素类B(26-216aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF

ICC/IF analysis of Cyclophilin B in Hep3B cells. The cell was stained with ATGA0545 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: HeLa cell lysate;;Lane 3.: Jurkate cell lysate;;Lane 4.: K562 cell lysate;;Lane 5.: 293T cell lysate;;Lane 6.: A549 cell lysate;;Lane 7.: MCF7 cell lysate;;Lane 8.: sk-ov-3 cell lysate;;Lane 9.: LnCap cell lysateThe Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human PPIB Protein;;Lane 2.: Recombinant human PPIA Protein;;Lane 3.: Recombinant human PPID Protein;;Lane 4.: Recombinant human PPIE Protein;;Lane 5.: Recombinant human PPIF Protein;;Lane 6.: Recombinant human PPIG Protein;;Lane 7.: Recombinant human PPIH Protein{ATGA0545-WB2.jpg}

人类

k2E2

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

人类

Cancer

This product was produced from tissue culture supernatant.

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P23284

26- 216aa, 26-216aa, AA408962, AA553318, AI844835, Cphn 2, Cphn2, Cphn-2, Cyclophilin B, Cyclophilin-B, CyclophilinB, CyP 20b, CyP-20b, CyP20b, CYP S1, CYP-S1, CYPS1, CYPB, CYP-S1: Cyclophilin-B, MGC14109, MGC2224, Peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, Peptidyl-prolyl cis-trans isomerase B, Peptidylprolyl isomerase B, PPIase, PPIB, Rotamase, S cyclophilin, SCYLP, ATGA0545-10ug, ATGA0545-20ug, ATGA0545-50ug, ATGA0545-100ug, ATGA0545-250ug, ATGA0545-500ug, ATGA0545-1mg, ATGA0545-010, ATGA0545-020, ATGA0545-050, ATGA0545-100, ATGA0545-250, ATGA0545-500, ATGA0545-01M

Mukadam AS, et al. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing. (Cell Mol Life Sci. 2018){https://pubmed.ncbi.nlm.nih.gov/29368044/}