产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类SUMO 2/3抗体
目录 :
ATGA0547
规格 :
50微升
价格 :
190美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT10F1
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类SUMO 2/3抗体
目录号(SKU号) :
ATGA0547
NCBI :
NP_008868
描述 :
SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family ofubiquitin-related proteins. 8-11 kDa that covalently modify various intracellular proteins. It resemblesubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of avariety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residuesafter the GG region of these precursors by SUMO-specific proteases in maturation is a prerequisite forsubsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recentstudies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical,biological consequences.
别称 :
ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, SuMO3, SuMO2, SMT3H2, SMT3H1, SMT3B, SMT3A, SMT3 suppressor of mif two 3 homolog 2, SMT3 homolog 2, SMT 3B, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), Small ubiquitin related modifier 2, Small ubiquitin like modifier 2, Sentrin2, MGC117191, HSMT3
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Tatsuya Ii, et al. (2007) DNA Repair (Amst), 6(11): 1679-1691.;;Zheng Xu, et al. (2005) Biochem J, 386(Pt 2): 325-330.;;Melchior F. (2000) Annu Rev Cell Dev Biol, 16: 591-626.;;
ab宿主 :
小鼠
抗体亚型 :
IgG2b kappa
免疫原 :
重组人类SUMO2(1-93aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of SUMO2/3 in HeLa cells. The cell was stained with ATGA0547 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: Jurkat cell lysate;;Lane 3.: K562 cell lysate;;Lane 4.: HL-60 cell lysateThe recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Recombinant human SUMO1 protein;;Lane 2.: Recombinant human SUMO2 protein;;Lane 3.: Recombinant human SUMO3 protein;;{ATGA0547-WB2.jpg}
反应对象 :
人类
克隆编号 :
AT10F1
Ab用途 :
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of SUMO2/3 in Jurkat cells. The cell was stained with ATGA0547 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
抗原物种 :
人类
基因类别 :
Epigenetics and Nuclear Signaling
Additional Comment Only :
This product was produced from tissue culture supernatant.
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P61956
内部搜索条款 :
HSMT 3, HSMT3, MGC117191, Sentrin 2, Sentrin2, Sentrin-2, Small ubiquitin like modifier 2, Small ubiquitin related modifier 2, Small ubiquitin-related modifier 2, Small ubiquitin-related modifier 2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), SMT 3B, SMT3 homolog 2, SMT3 suppressor of mif two 3 homolog 2, SMT3A, SMT3B, SMT3H1, SMT3H2, SuMO 3, SuMO2, SuMO-2, SuMO3, SuMO-3, ubiquitin like protein SMT3B.Small ubiquitin-related modifier 3, ATGA0547-10ug, ATGA0547-20ug, ATGA0547-50ug, ATGA0547-100ug, ATGA0547-250ug, ATGA0547-500ug, ATGA0547-1mg, ATGA0547-010, ATGA0547-020, ATGA0547-050, ATGA0547-100, ATGA0547-250, ATGA0547-500, ATGA0547-01M
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。