产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
磷酸甘油醛脱氢酶抗体
目录 :
ATGA0559
规格 :
50微升
价格 :
190美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AT4E8
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
磷酸甘油醛脱氢酶抗体
目录号(SKU号) :
ATGA0559
NCBI :
NP_002037
描述 :
Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis in the cytoplasm, it is involved in the translational control of gene expression in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.
别称 :
Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Chuang DM., et al, (2005) Annu Rev Pharmacol Toxicol. 45:269-90.;;Mazzola JL., et al, (2002) Neurotoxicology. 23(4-5):603-9.;;Sirover MA. (1997) J Cell Biochem. 66(2):133-40.
ab宿主 :
小鼠
抗体亚型 :
IgG2b kappa
免疫原 :
磷酸甘油醛脱氢酶来自兔肌肉
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF
IF Comment And Pic :
ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0559 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: MCF7 cell lysate;;Lane 3.: Jurkat cell lysate;;Lane 4.: K562 cell lysate;;Lane 5.: A431 cell lysate;;Lane 6.: LNCap cell lysate;;Lane 7.: Ramos cell lysateThe tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probedwith anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse Brain tissue lysate;;Lane 2.: Mouse Spleen tissue lysate;;Lane 3.: Mouse Eye tissue lysate;;Lane 4.: Mouse Muscle tissue lysate{ATGA0559-WB2.jpg}The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system.;;Lane 1.: Anti-GAPDH monoclonal antibody (1:100,000);;Lane 2.: Anti-GAPDH monoclonal antibody (1:50,000);;Lane 3.: Anti-GAPDH monoclonal antibody (1:10,000);;Lane 4.: Anti-GAPDH monoclonal antibody (1:1,000);;{ATGA0559-WB3.jpg}
克隆编号 :
AT4E8
Ab用途 :
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
基因类别 :
Cancer
Additional Comment Only :
This product was produced from tissue culture supernatant.
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
P04406
内部搜索条款 :
wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。