产品简要
公司名称 :
NKMAX
产品类型 :
抗体
产品名称 :
人类BHMT抗体
目录 :
ATGA0584
规格 :
50微升
价格 :
190美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3D6
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
产品信息
产品类型 :
抗体
产品名 :
人类BHMT抗体
目录号(SKU号) :
ATGA0584
NCBI :
NP_001704
描述 :
Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.
别称 :
三甲铵乙内酯-高半胱氨酸-S-转甲基酶1, BHMT1, 甜菜碱同型半胱氨酸甲基转移酶
浓度 :
1mg/ml (determined by BCA assay)
配方 :
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
储存 :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
一般参考 :
Mckeever MP et al.,(1991) Clin Sci 81(4):551-556.;;Norihiko F et al., (2001) Biochem J 129:313-320.;;Francisco G et al., (2005) Biochem J 391:589-599.;;Catherine M et al., (2005) Biochem J 392:443-448.;;Janet D. S et al., (2006) Biochem J 395:363-371.
ab宿主 :
小鼠
抗体亚型 :
IgG2a kappa
免疫原 :
重组人类BHMT(1-406aa)纯化来自大肠杆菌
应用 :
酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪
IF Comment And Pic :
ICC/IF analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment and Pic :
The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Mouse Liver Tissue lysate
反应对象 :
人类
克隆编号 :
3D6
Ab用途 :
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
FACS Comment and Pic :
Flow cytometry analysis of BHMT in Hep3B cells. The cell was stained with ATGA0584 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
抗原物种 :
人类
基因类别 :
代谢
Additional Comment Only :
This product was produced from tissue culture supernatant.
共轭 :
未共轭
Clonality Mono VS Poly :
单克隆
Ab纯化评论 :
By protein-A affinity chromatography
产品类型名 :
单克隆抗体
UniProt No. :
Q93088
内部搜索条款 :
甜菜碱同型半胱氨酸甲基转移酶, 三甲铵乙内酯-高半胱氨酸-S-转甲基酶, 三甲铵乙内酯-高半胱氨酸-S-转甲基酶1, 甜菜碱同型半胱氨酸甲基转移酶, 甜菜碱同型半胱氨酸甲基转移酶甜菜碱同型半胱氨酸甲基转移酶, 三甲铵乙内酯-高半胱氨酸-S-转甲基酶1, BHMT, BHMT(甜菜碱同型半胱氨酸甲基转移酶), BHMT1, EC 2.1。 1.5, ATGA0584-10微克, ATGA0584-20微克, ATGA0584-50微克, ATGA0584-100微克, ATGA0584-250微克, ATGA0584-500微克, ATGA0584-1毫克, ATGA0584-010, ATGA0584-020, ATGA0584-050, ATGA0584-100, ATGA0584-250, ATGA0584-500, ATGA0584-01M
更多信息或购买 :
公司信息
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
公司总部: 韩国
NKMAX有限公司是一家生物技术公司,制造和供应独特的优质蛋白质和抗体以供应世界各地的研究人员和零售商。