蛋白

重组人类血管内皮生长因子121/血管内皮生长因子A蛋白

ATGP0282

20微克

80美元

重组人类血管内皮生长因子121/血管内皮生长因子A蛋白

207-327aa

ATGP0282

NP_001020541.2

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF

0.5毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含10% 甘油

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54(1):16-24.;; Neufeld G., et al. (1999) FASEB J. 13(1):9-22.;;

> 95% by SDS-PAGE

措施在a细胞增殖检测美国HUVEC人类脐静脉内皮细胞 。 ED50范围≤4ng/毫升

SDS-PAGE, Bioactivity

人类

激素&细胞因子

14.2 kDa (122aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/ml.

MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR

Non-Tagged

大肠杆菌

重组蛋白

P15692

VEGF121, ATGP0282-10微克, ATGP0282-20微克, ATGP0282-50微克, ATGP0282-100微克, ATGP0282-250微克, ATGP0282-500微克, ATGP0282-1毫克, ATGP0282-10, ATGP0282-20, ATGP0282-50, ATGP0282-100, ATGP0282-250, ATGP0282-500, ATGP0282-1