蛋白

重组人类ATP5D蛋白

ATGP1099

10微克

240美元

重组人类ATP5D蛋白

23-168aa

ATGP1099

NP_001678

ATP5D, also known as F-ATPase delta subunit, catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This protein is the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. Recombinant human ATP5D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

ATP synthase subunit delta mitochondrial, ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit

0.25毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含20% 甘油, 0.1M氯化钠

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jordan E.M., et al. (1992) Biochim. Biophys. Acta 1130:123-126;; Grimwood J., et al. (2004) Nature. 428:529-535

> 95% by SDS-PAGE

十二烷基磺酸钠-PAGE

人类

代谢

17.3 kDa (167aa) confirmed by MALDI-TOF

MGSSHHHHHH SSGLVPRGSH MAEAAAAPAA ASGPNQMSFT FASPTQVFFN GANVRQVDVP TLTGAFGILA HVPTLQVLR PGLVVVHAED GTTSKYFVSS GSIAVNADSS VQLLAEEAVT LDMLDLGAAK ANLEKAQAEL VGTADEATRA EIQIRIEANE ALVKALE

多聚组氨酸标签

大肠杆菌

重组蛋白

P30049

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