蛋白

重组人类血管内皮生长因子165蛋白

ATGP1248

20微克

80美元

重组人类血管内皮生长因子165蛋白

207-371aa

ATGP1248

NP_001020539.2

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Vascular endothelial growth factor A isoform d, VPF, VEGF, VEGF-A

0.25毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值7.5)5mM DTT, 50% 甘油, 200mM氯化钠, 2mM乙二胺四乙酸

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54(1):16-24. ;; Neufeld G., et al. (1999) FASEB J. 13(1):9-22.

> 90% by SDS-PAGE

十二烷基磺酸钠-PAGE

人类

激素&细胞因子

21.4 kDa (186aa) conformed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

APMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

多聚组氨酸标签

大肠杆菌

重组蛋白

P15692

VEGF165, ATGP1248-10微克, ATGP1248-20微克, ATGP1248-50微克, ATGP1248-100微克, ATGP1248-250微克, ATGP1248-500微克, ATGP1248-1毫克, ATGP1248-10, ATGP1248-20, ATGP1248-50, ATGP1248-100, ATGP1248-250, ATGP1248-500, ATGP1248-1