蛋白

重组人类MYL12B蛋白

ATGP1577

20微克

80美元

重组人类MYL12B蛋白

1-172aa

ATGP1577

NP_001138417

Myosin, light chain 12B, regulatory, also known as MYL12B, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL12B regulates contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases Actin-activated myosin ATPase activity, thereby regulating contractile activity. Recombinant human MYL12B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myosin light chain 12B regulatory, Myosin, light chain 12B regulatory, MLC-B, MRLC2

1毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含0.2M氯化钠, 40% 甘油, 2mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kumar C C., et al. (1989) Biochemistry. 28:4027-4035.;; Kolodney M S., et al. (1999) J Physiol. 515: 87-92.

> 90% by SDS-PAGE

十二烷基磺酸钠-PAGE

人类

Actin & Myosin Related

22.3 kDa (196aa) confirmed by MALDI-TOF

MSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD

多聚组氨酸标签

大肠杆菌

重组蛋白

O14950

MYL12B, Myosin light chain 12B regulatory, Myosin, light chain 12B regulatory, MLC-B, MRLC2,ATGP1577-10ug,ATGP1577-20ug,ATGP1577-50ug,ATGP1577-100ug,ATGP1577-250ug,ATGP1577-500ug,ATGP1577-1mg,ATGP1577-10,ATGP1577-20,ATGP1577-50,ATGP1577-100,ATGP1577-250,ATGP1577-500,ATGP1577-1