蛋白

Recombinant human NEU-1/Sialidase-1 protein

ATGP1685

20微克

105美元

Recombinant human NEU-1/Sialidase-1 protein

48-415aa

ATGP1685

NP_000425

NEu1 is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A. Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red -myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. Recombinant human NEu1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sialidase 1 (lysosomal sialidase), NANH, NEu, SIAL1

0.25毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含0.15M氯化钠, 10% 甘油, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Abdulkhalek,S., et al. (2011) J. Biol. Chem. 286 (42), 36532-36549

> 85% by SDS-PAGE

十二烷基磺酸钠-PAGE

人类

代谢

42.9 kDa (393aa) confirmed by MALDI-TOF

GSSHHHHHH SSGLVPRGSH MGSHMENDFG LVQPLVTMEQ LLWVSGRQIG SVDTFRIPLI TATPRGTLLA FAEARKMSSS DEGAKFIALR RSMDQGSTWS PTAFIVNDGD VPDGLNLGAV VSDVETGVVF LFYSLCAHKA GCQVASTMLV WSKDDGVSWS TPRNLSLDIG TEVFAPGPGS GIQKQREPRK GRLIVCGHGT LERDGVFCLL SDDHGASWRY GSGVSGIPYG QPKQENDFNP DECQPYELPD GSVVINARNQ NNYHCHCRIV LRSYDACDTL RPRDVTFDPE LVDPVVAAGA VVTSSGIVFF SNPAHPEFRV NLTLRWSFSN GTSWRKETVQ LWPGPSGYSS LATLEGSMDG EEQAPQLYVL YEKGRNHYTE SISVAKISVY GTL

多聚组氨酸标签

大肠杆菌

重组蛋白

Q99519

NEU1, Sialidase1, Sialidase 1 (lysosomal sialidase), NANH, NEu, SIAL1,ATGP1685-10ug,ATGP1685-20ug,ATGP1685-50ug,ATGP1685-100ug,ATGP1685-250ug,ATGP1685-500ug,ATGP1685-1mg,ATGP1685-10,ATGP1685-20,ATGP1685-50,ATGP1685-100,ATGP1685-250,ATGP1685-500,ATGP1685-1