蛋白

重组人类RNA聚合酶i, II, 和III亚单位RPABC1/POLR2E蛋白

ATGP1843

20微克

80美元

重组人类RNA聚合酶i, II, 和III亚单位RPABC1/POLR2E蛋白

1-210aa

ATGP1843

NP_002686

POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

RNA聚合酶i, II, 和III亚单位RPABC1/POLR2E蛋白

1毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含0.1M氯化钠, 10% 甘油, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453;; Dorjsuren D., et al. (1998) Mol. Cell. Biol. 18:7546-7555;;

> 90% by SDS-PAGE

十二烷基磺酸钠-PAGE

人类

Epigenomics (Transcription & Translation)

27 kDa (233aa) confirmed by MALDI-TOF

MDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVT ELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ

多聚组氨酸标签

大肠杆菌

重组蛋白

P19388

POLR2E, DNA指导的RNA聚合酶 I II和III亚单位RPABC1, DNA指导的RNA聚合酶 I, II, 和III亚单位RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4, ATGP1843-10微克, ATGP1843-20微克, ATGP1843-50微克, ATGP1843-100微克, ATGP1843-250微克, ATGP1843-500微克, ATGP1843-1毫克, ATGP1843-10, ATGP1843-20, ATGP1843-50, ATGP1843-100, ATGP1843-250, ATGP1843-500, ATGP1843-1