蛋白

Recombinant human B-Raf protein

ATGP2924

20微克

105美元

Recombinant human B-Raf protein

432-766aa

ATGP2924

NP_004324.2

BRAF belongs to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. Recombinant human BRAF protein, fused to His-tag at N-terminus, was expressed in E. coli.

Serine/threonine-protein kinase B-raf, Serine/threonine-protein kinase B-raf, B-RAF1, BRAF1, NS7, RAFB1

0.25毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含10% 甘油

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Brennan D.F., et al. (2011) Nature 472:366-369

> 80% by SDS-PAGE

十二烷基磺酸钠-PAGE, 变性的

人类

凋亡

40.6 kDa (360aa)

SEDRN RMKTLGRRDS SDDWEIPDGQ ITVGQRIGSG SFGTVYKGKW HGDVAVKMLN VTAPTPQQLQ AFKNEVGVLR KTRHVNILLF MGYSTKPQLA IVTQWCEGSS LYHHLHIIET KFEMIKLIDI ARQTAQGMDY LHAKSIIHRD LKSNNIFLHE DLTVKIGDFG LATVKSRWSG SHQFEQLSGS ILWMAPEVIR MQDKNPYSFQ SDVYAFGIVL YELMTGQLPY SNINNRDQII FMVGRGYLSP DLSKVRSNCP KAMKRLMAEC LKKKRDERPL FPQILASIEL LARSLPKIHR SASEPSLNRA GFQTEDFSLY ACASPKTPIQ AGGYGAFPVH

多聚组氨酸标签

大肠杆菌

重组蛋白

P15056

B-Raf, Serine/threonine-protein kinase B-raf, Serine/threonine-protein kinase B-raf, B-RAF1, BRAF1, NS7, RAFB1,ATGP2924-10ug,ATGP2924-20ug,ATGP2924-50ug,ATGP2924-100ug,ATGP2924-250ug,ATGP2924-500ug,ATGP2924-1mg,ATGP2924-10,ATGP2924-20,ATGP2924-50,ATGP2924-100,ATGP2924-250,ATGP2924-500,ATGP2924-1