蛋白

重组人类肿瘤坏死因子甲蛋白

ATGP3152

10微克

80美元

重组人类肿瘤坏死因子甲蛋白

77-233aa

ATGP3152

NP_000585

TNF, also known as tumor necrosis factor, is cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation. Recombinant human TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

1mg/ml (determined by absorbance at 280nm)

液体。 磷酸盐缓冲液(pH值7.4)含10% 甘油

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Friedmann E. et al., (2006) Nat. Cell Biol. 8:843-848.;; Zhang XM. et al., (1992) J. Biol. Chem. 267:24069-24075.

> 90% by SDS-PAGE

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

SDS-PAGE, Bioactivity

人类

免疫学

18.1 kDa (163aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human TNF-alpha stimulates cell cytotoxicity in the L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH

多聚组氨酸标签

杆状病毒

重组蛋白

P01375

肿瘤坏死因子-a, 肿瘤坏死因子, DIF, 肿瘤坏死因子甲, TNFA, TNFSF2, ATGP3152-10微克, ATGP3152-20微克, ATGP3152-50微克, ATGP3152-100微克, ATGP3152-250微克, ATGP3152-500微克, ATGP3152-1毫克, ATGP3152-10, ATGP3152-20, ATGP3152-50, ATGP3152-100, ATGP3152-250, ATGP3152-500, ATGP3152-1

Kim JA, et al. Effects of impressic acid from Acanthopanax koreanum on NF-κB and PPARγ activities. (Arch Pharm Res. 2011){https://pubmed.ncbi.nlm.nih.gov/21910057/};;Kim YH, et al. Benzyl Glycosides from the Aerial Parts of Gynostemma laxum and Their NF-κB Inhibitory Activity in HepG2 Cells. (Bulletin of the Korean Chemical Society. 2011){https://doi.org/10.5012/bkcs.2011.32.10.3763};;Kim JA, et al. A new lupane-type triterpene from the seeds of Panax ginseng with its inhibition of NF-κB. (Arch Pharm Res. 2012){https://pubmed.ncbi.nlm.nih.gov/22553057/};;Nhiem NX, et al. Inhibition of nuclear transcription factor-κB and activation of peroxisome proliferator-activated receptors in HepG2 cells by cucurbitane-type triterpene glycosides from Momordica charantia. (J Med Food. 2012){https://pubmed.ncbi.nlm.nih.gov/22248180/};;Song SB, et al. Inhibition of TNF-α-mediated NF-κB Transcriptional Activity in HepG2 Cells by Dammarane-type Saponins from Panax ginseng Leaves. (J Ginseng Res. 2012){https://pubmed.ncbi.nlm.nih.gov/23717114/};;Liang C, et al. Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB. (J Ginseng Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23717159/};;Thao NP, et al. Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima. (Arch Pharm Res. 2014){https://pubmed.ncbi.nlm.nih.gov/23934574/};;Cho K, et al. Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/24596622/};;Li W, et al. Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/25143813/};;He F, et al. Antitumor effects of dammarane-type saponins from steamed Notoginseng. (Pharmacogn Mag. 2014){https://pubmed.ncbi.nlm.nih.gov/25210319/};;Thao NP, et al. NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum. (Bioorg Med Chem Lett. 2014){https://pubmed.ncbi.nlm.nih.gov/24852121/};;Yang SY, et al. NF-κB Activation and PPAR Transactivational Effects of a New Aliphatic Acid Amide from Pericarps of Zanthoxylum piperitum. (Bulletin of the Korean Chemical Society. 2014){https://doi.org/10.5012/bkcs.2014.35.8.2361};;Sun YN, et al. NF-κB Inhibitory Activities of Phenolic and Lignan Components from the Stems of Acanthopanax divaricatus var. albeofructus. (The Korean Society of Pharmacognosy. 2014){https://koreascience.kr/article/JAKO201402852974800.page};;Sun YN, et al. Isolation and identification of chromone and pyrone constituents from Aloe and their anti-inflammatory activities. (Journal of Functional Foods. 2015){https://doi.org/10.1016/j.jff.2015.12.026};;Sun YN, et al. Nuclear Factor kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. (Pharmacogn Mag. 2016){https://pubmed.ncbi.nlm.nih.gov/27019559/};;Sun YN, et al. Nuclear Factor kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. (Nat Prod Res. 2016){https://pubmed.ncbi.nlm.nih.gov/26647286/};;Kwak MS, et al. A new phenolic derivative with soluble epoxide hydrolase and nuclear factor-kappaB inhibitory activity from the aqueous extract of Acacia catechu. (Redox Biol. 2019){https://pubmed.ncbi.nlm.nih.gov/31026770/}