蛋白

重组小鼠IL-12 p70蛋白

ATGP3739

10微克

105美元

重组小鼠IL-12 p70蛋白

23-335aa(p40)/23-215aa(p35)

ATGP3739

NP_001290173(p40)/NP_032377(p35)

IL12b/IL12a, also known as interleukin 12 (subunit beta/alpha), is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. It is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. As it is associated with IL23A to form the IL-23 interleukin, it induces autoimmune inflammation and is responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Recombinant mouse IL12b/IL12a protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin-12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2

0.5毫克/毫升(由布拉德福德Bradford检测确定)

液体。 磷酸盐缓冲液(pH值7.4)含10% 甘油

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Zhang X., et al, (2015) J Immunol. 195:1665-1675.;; Zamani A., et al, (2017) Immune Netw. 17:186-191.

> 95% by SDS-PAGE

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 for this effect is less or equal to 10ng/ml.

SDS-PAGE, Bioactivity

小鼠

激素&细胞因子

35.7 kDa (p40, 313aa);; 22.5 kDa (p35, 199aa);;The heterodimeric Il12 has a predicted Molecular weight of 58.3 kDa (512aa).;;Molecular weight on SDS-PAGE will appear higher

< 1 EU per 1ug of protein (determined by LAL method)

Mouse IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less or equal to 10 ng/ml.

IL12B(p40);;MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS;;IL12A(p35);;RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA

多聚组氨酸标签

杆状病毒

Met23(p40)/Arg23(p35)

重组蛋白

P43432(p40)/P43431(p35)

IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin-12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2, IL12, IL-12, Interleukin12, Interleukin 12, IL 12, Interleukin 12 alpha chain, Interleukin 12 beta chain, ATGP3739-10ug, ATGP3739-20ug, ATGP3739-50ug, ATGP3739-100ug, ATGP3739-250ug, ATGP3739-500ug, ATGP3739-1mg, ATGP3739-010, ATGP3739-020, ATGP3739-050, ATGP3739-100, ATGP3739-250, ATGP3739-500, ATGP3739-01M