抗体

人类Wnt 3a抗体

AWN0924

50微升

170美元

单克隆

小鼠

未共轭

3A6

免疫印迹, 酶联免疫吸附测定, 免疫组化

人类Wnt 3a抗体

AWN0924

NP_149122

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors.

Wnt3a, Wingless-type MMTV integration site family, member 3A

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Pan W., et al. (2008) Science. 321(5894):1350-1353.;; Katoh M., et al. (2002) Int J Oncol. 20(2):373-377.;; Smolich B D., et al. (1993) Mol Biol Cell. 4(12):1267-1275.

小鼠

IgG2a kappa

重组人类Wnt3a(19-352aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫组化

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Wnt3a antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: 293T cell lysate;; Lane 2.: Wnt3a Transfected 293T cell lysate

人类

3A6

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

人类

Development

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P56704

Wnt3a, AWN0924-10微克, AWN0924-20微克, AWN0924-50微克, AWN0924-100微克, AWN0924-250微克, AWN0924-500微克, AWN0924-1毫克, AWN0924-10, AWN0924-20, AWN0924-50, AWN0924-100, AWN0924-250, AWN0924-500, AWN0924-1

Chen F, et al. Curcumin increased the differentiation rate of neurons in neural stem cells via wnt signaling in vitro study. (J Surg Res. 2014){https://pubmed.ncbi.nlm.nih.gov/25033705/};;Gong SJ, et al. Upregulation of PP2Ac predicts poor prognosis and contributes to aggressiveness in hepatocellular carcinoma. (Cancer Biol Ther. 2016){https://pubmed.ncbi.nlm.nih.gov/26618405/};;Pan LH, et al. Oncogenic Wnt3a expression as an estimable prognostic marker for hepatocellular carcinoma. (World J Gastroenterol. 2016){https://pubmed.ncbi.nlm.nih.gov/27076768/};;Bai D, et al. Attenuation of Focal Adhesion Kinase Reduces Lipopolysaccharide-Induced Inflammation Injury through Inactivation of the Wnt and NF-κB Pathways in A549 Cells. (Biochemistry (Mosc). 2017){https://pubmed.ncbi.nlm.nih.gov/28371601/};;Meng X, et al. Heterogeneous nuclear ribonucleoprotein A1 interacts with microRNA-34a to promote chondrogenic differentiation of mesenchymal stem cells. (Am J Transl Res. 2017){https://pubmed.ncbi.nlm.nih.gov/28469782/};;Xiang T, et al. Oxidored-nitro domain-containing protein 1 promotes liver fibrosis by activating the Wnt/β-catenin signaling pathway in vitro. (Mol Med Rep. 2017){https://pubmed.ncbi.nlm.nih.gov/28791396/};;Li X, et al. Leptin differentially regulates endochondral ossification in tibial and vertebral epiphyseal plates. Cell Biol Int. 2018{https://pubmed.ncbi.nlm.nih.gov/28980745/};;Xue C, et al. β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. (Biosci Rep. 2018){https://pubmed.ncbi.nlm.nih.gov/29358311/};;Wang L, et al. Expression of hepatic Wnt5a and its clinicopathological features in patients with hepatocellular carcinoma. (Hepatobiliary Pancreat Dis Int. 2018){https://pubmed.ncbi.nlm.nih.gov/29709351/};;Liu SQ, et al. Propofol Inhibits Proliferation, Migration, Invasion and Promotes Apoptosis Through Down-Regulating miR-374a in Hepatocarcinoma Cell Lines. (Cell Physiol Biochem. 2018){https://pubmed.ncbi.nlm.nih.gov/30257238/}