蛋白

重组人类亲环素A/PPIA蛋白

CYP0702

20微克

70美元

重组人类亲环素A/PPIA蛋白

1-165aa

CYP0702

NP_066953

Cyclophilin A (also known as Peptidylprolyl isomerase A, PPIA) encodes a member of the peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. Cyclophilin A is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. Recombinant human PPIA, was expressed in E. coli and purified by using conventional chromatography techniques.

Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, Cyclophilin A, Cyclosporin A binding protein, Peptidyl prolyl cis trans isomerase A, PPIA, PPIA protein, PPIase A, Rotamase A, RotamaseA, T cell cyclophilin.

1毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含20mM氯化钠, 0.5mM DTT, 10% 甘油

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Holzman, T.F., et al, (1991). J. Biol. Chem. 266(4): 2474-2479;; Berthoux L , et al, (2005). Proc Natl Acad Sci u S A. 102(41):14849-53;;

> 95% by SDS-PAGE

Specific activity is > 650nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

十二烷基磺酸钠-PAGE, 酶活性

人类

细胞周期

20kDa (185aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 650 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE

多聚组氨酸标签

大肠杆菌

重组蛋白

P62937

Cyclophilin A, PPIA, Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, Cyclophilin A, Cyclosporin A binding protein, Peptidyl prolyl cis trans isomerase A, PPIA, PPIA protein, PPIase A, Rotamase A, RotamaseA, T cell cyclophilin.,CYP0702-10ug,CYP0702-20ug,CYP0702-50ug,CYP0702-100ug,CYP0702-250ug,CYP0702-500ug,CYP0702-1mg,CYP0702-10,CYP0702-20,CYP0702-50,CYP0702-100,CYP0702-250,CYP0702-500,CYP0702-1

Gallay PA, et al. The Novel Cyclophilin Inhibitor CPI-431-32 Concurrently Blocks HCV and HIV-1 Infections via a Similar Mechanism of Action. (PLoS One. 2015){https://www.ncbi.nlm.nih.gov/pubmed/26263487};;Gallay PA, et al. Characterization of the Anti-HCV Activities of the New Cyclophilin Inhibitor STG-175. (PLoS One. 2016){https://www.ncbi.nlm.nih.gov/pubmed/27104614};;Kim K, et al. Recombinant human Cyclophilin A stimulates hair follicle cells via Wnt/β-catenin signaling pathway. (Biotechnol Lett. 2019){https://pubmed.ncbi.nlm.nih.gov/31655924/};;Tsuda T, et al. Rho-associated protein kinase and cyclophilin a are involved in inorganic phosphate-induced calcification signaling in vascular smooth muscle cells. (J Pharmacol Sci. 2020){https://pubmed.ncbi.nlm.nih.gov/31882204/}