蛋白

重组人类EGF蛋白

EGF0501

20微克

25美元

重组人类EGF蛋白

971-1023aa

EGF0501

NP_001954.2

Recombinant human epidermal growth factor (EGF) is a 6. 3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Epidermal growth factor, Pro-epidermal growth factor, urogastrone, Epidermal growth factor, EGF, epidermal growth factor (beta-urogastrone)

1毫克/毫升(由布拉德福德Bradford检测确定)

液体。 磷酸盐缓冲液(pH值7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Riese., et al (1998 ) Bioessays. 20: 41-48.;; Cohen S., (1983) Cancer. 15: 1787-1791. ;; Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.;;

> 95% by SDS-PAGE

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is less or equal to 0.1ng/ml.

SDS-PAGE, Bioactivity

人类

激素&细胞因子

6.3 kDa (54aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range is less or equal to 0.1 ng/ml.

MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

Non-Tagged

大肠杆菌

重组蛋白

P01133

EGF, EGF0501-10微克, EGF0501-20微克, EGF0501-50微克, EGF0501-100微克, EGF0501-250微克, EGF0501-500微克, EGF0501-1毫克, EGF0501-10, EGF0501-20, EGF0501-50, EGF0501-100, EGF0501-250, EGF0501-500, EGF0501-1

Tazawa H, et al. Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells. (Exp Cell Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23948305/};;Park SY, et al. DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling. (Biochim Biophys Acta. 2015){https://pubmed.ncbi.nlm.nih.gov/26239118/};;Okuda K, et al. Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis. (Nat Commun. 2023){https://pubmed.ncbi.nlm.nih.gov/36739439/}