蛋白

重组人类HSP60蛋白

HSP0802

20微克

80美元

重组人类HSP60蛋白

1-573aa

HSP0802

NP_002147.2

Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E. coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding. Recombinant human HSP60, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Heat shock 60kDa protein 1, CPN60, GroEL, HSP65, SPG13, HuCHA60, Heat shock 60kDa protein 1, HSP60, Heat shock 60kDa protein 1 60 kDa chaperonin, GroEL, E, coli, homolog of, 60 kDa heat shock protein mitochondrial, 60kDa, cb863, Chaperonin, Chaperonin 60, Chaperonin, 60-KD, CPN 60, fa04a05, fb22d10, fi27b05, GroEL Homolog, Heat shock 60kD protein 1 (chaperonin), Heat shock 60kD protein 1 chaperonin, heat shock 60kDa protein 1 (chaperonin), Heat shock protein 1 (chaperonin), Heat Shock Protein 60, Heat shock protein 65, HLD4, Hsp 60, HSP 65, HSPD 1, HSPD1, HuCHA60, id:ibd2197, Spastic paraplegia 13 Mitochondrial matrix protein P1, P60 lymphocyte protein, sb:cb144, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (autosomal dominant), SPG 13, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05.

1毫克/毫升(由布拉德福德Bradford检测确定)

液体。 25mM三羟甲基氨基甲烷氯化氢缓冲液(pH值7.5)含100mM氯化钠, 5mM DTT, 10% 甘油

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Cheng MY., et al. (1990), Nature. 348: 455- 458;; Ghosh JC., et al. (2008), J Biol Chem. Feb 22; 283(8):5188-94

> 95% by SDS-PAGE

十二烷基磺酸钠-PAGE

人类

热休克蛋白

63.2 kDa (593aa)

MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALM LQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSK PVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG神经胶质成熟因子

多聚组氨酸标签

大肠杆菌

重组蛋白

P10809

HSP60, Heat shock 60kDa protein 1, CPN60, GroEL, HSP65, SPG13, HuCHA60, Heat shock 60kDa protein 1, HSP60, Heat shock 60kDa protein 1 60 kDa chaperonin, GroEL, E, coli, homolog of, 60 kDa heat shock protein mitochondrial, 60kDa, cb863, Chaperonin, Chaperonin 60, Chaperonin, 60-KD, CPN 60, fa04a05, fb22d10, fi27b05, GroEL Homolog, Heat shock 60kD protein 1 (chaperonin), Heat shock 60kD protein 1 chaperonin, heat shock 60kDa protein 1 (chaperonin), Heat shock protein 1 (chaperonin), Heat Shock Protein 60, Heat shock protein 65, HLD4, Hsp 60, HSP 65, HSPD 1, HSPD1, HuCHA60, id:ibd2197, Spastic paraplegia 13 Mitochondrial matrix protein P1, P60 lymphocyte protein, sb:cb144, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (autosomal dominant), SPG 13, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05.,HSP0802-10ug,HSP0802-20ug,HSP0802-50ug,HSP0802-100ug,HSP0802-250ug,HSP0802-500ug,HSP0802-1mg,HSP0802-10,HSP0802-20,HSP0802-50,HSP0802-100,HSP0802-250,HSP0802-500,HSP0802-1

Kaiser F, et al. Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. (Cell Stress Chaperones. 2014){https://pubmed.ncbi.nlm.nih.gov/23775284/};;Vilasi S,et al. Human Hsp60 with its mitochondrial import signal occurs in solution as heptamers and tetradecamers remarkably stable over a wide range of concentrations. (PLoS One. 2014){https://pubmed.ncbi.nlm.nih.gov/24830947/};;Ricci C, et al. Stability and disassembly properties of human naïve Hsp60 and bacterial GroEL chaperonins. (Biophys Chem. 2016){https://pubmed.ncbi.nlm.nih.gov/26259786/};;Ricci C, et al. Investigation on different chemical stability of mitochondrial Hsp60 and its precursor. (Biophys Chem. 2017){https://pubmed.ncbi.nlm.nih.gov/28774748/};;Kaiser F, et al. Association between circulating levels of heat-shock protein 27 and aggressive periodontitis. 9Cell Stress Chaperones. 2018){https://pubmed.ncbi.nlm.nih.gov/29766408/}