蛋白

重组人类丝氨酸蛋白酶抑制剂E1/PAI-1蛋白

PAI0701

20微克

80美元

重组人类丝氨酸蛋白酶抑制剂E1/PAI-1蛋白

24-402aa

PAI0701

NP_000593

Plasminogen activator inhibitor-1 (PAI-1), a member of the serine protease inhibitor (serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

纤溶酶原激活物抑制因子1, PLANH1, SERPINE1, PAI1, PLANH1, 纤溶酶原激活物抑制因子1, 纤溶酶原激活物抑制因子1

1毫克/毫升(由布拉德福德Bradford检测确定)

Liquid in. 50mM NaAc (pH 5.5) containing 10% glycerol, 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Gorlatova NV., et al.(2007) J Biol Chem. 282(12):9288-96.;; Renckens R., et al. (2006) J Immunol. 177(11):8171-6.;;

> 95% by SDS-PAGE

The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

十二烷基磺酸钠-PAGE, 酶活性

人类

酶&蛋白酶

45 kDa (400aa)

The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP

多聚组氨酸标签

大肠杆菌

重组蛋白

P05121

丝氨酸蛋白酶抑制剂E1, PAI1, 纤溶酶原激活物抑制因子1, PLANH1, SERPINE1, PAI1, PLANH1, 纤溶酶原激活物抑制因子1, 纤溶酶原激活物抑制因子1, PAI0701-10微克, PAI0701-20微克, PAI0701-50微克, PAI0701-100微克, PAI0701-250微克, PAI0701-500微克, PAI0701-1毫克, PAI0701-10, PAI0701-20, PAI0701-50, PAI0701-100, PAI0701-250, PAI0701-500, PAI0701-1

Cho KS, et al. Valproic acid induces astrocyte-dependent neurite outgrowth from cultured rat primary cortical neuron via modulation of tPA/PAI-1 activity. (Glia. 2013){https://pubmed.ncbi.nlm.nih.gov/23378038/}