蛋白

重组人类肿瘤坏死因子甲蛋白

TNF0501

20微克

55美元

重组人类肿瘤坏死因子甲蛋白

77-233aa

TNF0501

NP_000585.2

Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17. 5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

1毫克/毫升(由布拉德福德Bradford检测确定)

液体。 磷酸盐缓冲液(pH值7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Chicoine M.R., et al. (1997), J. Neurooncol. 35: 249-57;; Ruddle N.H., et al. (1992), Curr. Opin. Immunol. 4: 327-32;; Wingfield P., et al. (1987), FEBS Lett. 211:179-184;;

> 95% by SDS-PAGE

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

SDS-PAGE, Bioactivity

人类

激素&细胞因子

17.5 kDa (158aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Non-Tagged

大肠杆菌

重组蛋白

P01375

TNF-a, Tumor necrosis factor alpha, TNFA, TNFSF2, DIF (differentiation inducing factor ), CACHECTIN, DIF, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor alpha, TNF, tumor necrosis factor (TNF superfamily, member 2), TNF superfamily member 2,TNF0501-10ug,TNF0501-20ug,TNF0501-50ug,TNF0501-100ug,TNF0501-250ug,TNF0501-500ug,TNF0501-1mg,TNF0501-10,TNF0501-20,TNF0501-50,TNF0501-100,TNF0501-250,TNF0501-500,TNF0501-1

Kim JA, et al. Effects of impressic acid from Acanthopanax koreanum on NF-κB and PPARγ activities. (Arch Pharm Res. 2011){https://pubmed.ncbi.nlm.nih.gov/21910057/};;Kim YH, et al. Benzyl Glycosides from the Aerial Parts of Gynostemma laxum and Their NF-κB Inhibitory Activity in HepG2 Cells. (Bulletin of the Korean Chemical Society. 2011){https://doi.org/10.5012/bkcs.2011.32.10.3763};;Kim JA, et al. A new lupane-type triterpene from the seeds of Panax ginseng with its inhibition of NF-κB. (Arch Pharm Res. 2012){https://pubmed.ncbi.nlm.nih.gov/22553057/};;Nhiem NX, et al. Inhibition of nuclear transcription factor-κB and activation of peroxisome proliferator-activated receptors in HepG2 cells by cucurbitane-type triterpene glycosides from Momordica charantia. (J Med Food. 2012){https://pubmed.ncbi.nlm.nih.gov/22248180/};;Song SB, et al. Inhibition of TNF-α-mediated NF-κB Transcriptional Activity in HepG2 Cells by Dammarane-type Saponins from Panax ginseng Leaves. (J Ginseng Res. 2012){https://pubmed.ncbi.nlm.nih.gov/23717114/};;Liang C, et al. Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB. (J Ginseng Res. 2013){https://pubmed.ncbi.nlm.nih.gov/23717159/};;Thao NP, et al. Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima. (Arch Pharm Res. 2014){https://pubmed.ncbi.nlm.nih.gov/23934574/};;Cho K, et al. Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/24596622/};;Li W, et al. Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana. (Biomol Ther (Seoul). 2014){https://pubmed.ncbi.nlm.nih.gov/25143813/};;He F, et al. Antitumor effects of dammarane-type saponins from steamed Notoginseng. (Pharmacogn Mag. 2014){https://pubmed.ncbi.nlm.nih.gov/25210319/};;Thao NP, et al. NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum. (Bioorg Med Chem Lett. 2014){https://pubmed.ncbi.nlm.nih.gov/24852121/};;Yang SY, et al. NF-κB Activation and PPAR Transactivational Effects of a New Aliphatic Acid Amide from Pericarps of Zanthoxylum piperitum. (Bulletin of the Korean Chemical Society. 2014){https://doi.org/10.5012/bkcs.2014.35.8.2361};;Sun YN, et al. NF-κB Inhibitory Activities of Phenolic and Lignan Components from the Stems of Acanthopanax divaricatus var. albeofructus. (The Korean Society of Pharmacognosy. 2014){https://koreascience.kr/article/JAKO201402852974800.page};;Sun YN, et al. Isolation and identification of chromone and pyrone constituents from Aloe and their anti-inflammatory activities. (Journal of Functional Foods. 2015){https://doi.org/10.1016/j.jff.2015.12.026};;Sun YN, et al. Nuclear Factor kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. (Pharmacogn Mag. 2016){https://pubmed.ncbi.nlm.nih.gov/27019559/};;Sun YN, et al. Nuclear Factor kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. (Nat Prod Res. 2016){https://pubmed.ncbi.nlm.nih.gov/26647286/};;Kwak MS, et al. A new phenolic derivative with soluble epoxide hydrolase and nuclear factor-kappaB inhibitory activity from the aqueous extract of Acacia catechu. (Redox Biol. 2019){https://pubmed.ncbi.nlm.nih.gov/31026770/}