蛋白

重组人类血管内皮生长因子121/血管内皮生长因子A蛋白

VEG0901

10微克

105美元

重组人类血管内皮生长因子121/血管内皮生长因子A蛋白

207-327aa

VEG0901

NP_001020541

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF

0.5毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含10% 甘油

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yasuji ueda., et al (2004) Cancer Chemother Pharmacol. 54(1):16-24;; Neufeld G., et all(1999) FASEB J. 13(1):9-22;;

> 90% by SDS-PAGE

措施在a细胞增殖检测美国HUVEC人类脐静脉内皮细胞 。 ED50范围≤12ng/毫升

SDS-PAGE, Bioactivity

人类

Cancer

16.3 kDa (142aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤12 ng/ml.

APMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR

多聚组氨酸标签

大肠杆菌

重组蛋白

P15692

VEGF121, VEG0901-10微克, VEG0901-20微克, VEG0901-50微克, VEG0901-100微克, VEG0901-250微克, VEG0901-500微克, VEG0901-1毫克, VEG0901-10, VEG0901-20, VEG0901-50, VEG0901-100, VEG0901-250, VEG0901-500, VEG0901-1