MHC Class I Antibody (OX18)
Novus Biologicals
目录: NB120-6405
小鼠 单克隆 (OX18)
反应物种: 人类, 大鼠
应用: 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 中和反应, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 7

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
规格: 0.1 mg (also 0.025 mg)
价格: 279美元
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QA1b抗体(6A8.6F10.1A6)
Novus Biologicals
目录: NBP2-26649
小鼠 单克隆 (6A8.6F10.1A6)
反应物种: 人类, 小鼠
应用: 免疫印迹, 中和反应, 免疫沉淀, 流式细胞仪
文章摘录数: 3

Western Blot: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis of QA1b in mouse thymus tissue lysate using NBP2-26649 at 2 ug/mL.

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Analysis using the Biotin conjugate of NBP2-26649. Staining of resting and ConA activated (5ug/ml for 72 hr) BALB/C splenocytes using 0.5 ug of Qa-1b biotinylated antibody . CD3 positive cells were gated. Streptavidin - PE (SA-PE) was usedas secondary antibody. a) SA-PE resting cells. b) SA-PE ConA activated cells. c) Qa-1b resting cells. d) Qa-1b ConA activated cells.

Flow Cytometry: QA1b Antibody (6A8.6F10.1A6) [NBP2-26649] - Flow cytometric analysis of QA1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) NBP2-26649 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/10^6 cells. A shift is seen in only a small percentage of cells.
规格: 0.1 mg (also 0.025 mg)
价格: 329美元
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MHC Class I Antibody (ERMP42)
Novus Biologicals
目录: NB120-15680
大鼠 单克隆 (ERMP42)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 流式细胞仪, 免疫组化-冰冻切片
文章摘录数: 3
规格: 0.05毫克
价格: 279美元
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MHC Class I Antibody (2G5)
Novus Biologicals
目录: NB100-65938
小鼠 单克隆 (2G5)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫沉淀, 流式细胞仪
文章摘录数: 3
规格: 0.125 mg (also 0.025 mg)
价格: 329美元
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MHC Class I Antibody (JF10-38)
Novus Biologicals
目录: NBP2-66946
domestic rabbit 单克隆 (JF10-38)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: Raji cell lysate
规格: 100微升
价格: 379美元
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MHC Class I Antibody (OX18) - Azide and BSA Free
Novus Biologicals
目录: NBP2-80848
小鼠 单克隆 (OX18)
反应物种: 人类, 大鼠
应用: 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 中和反应, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
规格: 0.1毫克
价格: 279美元
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MHC Class I Antibody (H58A)
Novus Biologicals
目录: NBP2-61002
小鼠 单克隆 (H58A)
反应物种: 人类
应用: 免疫组化, 流式细胞仪, 免疫组化-冰冻切片

Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.
规格: 0.1毫克
价格: 279美元
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MHC Class I Antibody (MEM-E/06)
Novus Biologicals
目录: NB500-307
小鼠 单克隆 (MEM-E/06)
反应物种: 人类
应用: 免疫组化, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片

Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).
规格: 0.1毫克
价格: 309美元
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