产品简要
信息索取 :
公司名称 :
Novus Biologicals
其他品牌 :
IMGENEX
产品类型 :
抗体
产品名称 :
Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488]
目录 :
NB100-123L
规格 :
0.1毫升
价格 :
399美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
H1alpha67
反应物种 :
人类, 小鼠, 大鼠, ,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 7
已发表的文献中的应用方法/种属/稀释度参考文献
  • 免疫印迹; 小鼠; 1:500; 图 5
  • 免疫细胞化学; 小鼠; 1:100; 图 1
Schokrpur S, Hu J, Moughon D, Liu P, Lin L, Hermann K, et al. CRISPR-Mediated VHL Knockout Generates an Improved Model for Metastatic Renal Cell Carcinoma. Sci Rep. 2016;6:29032 pubmed 出版商
Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell W, Tan A, et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis. 2017;6:e287 pubmed 出版商
Lee S, Frattini V, Bansal M, Castano A, Sherman D, Hutchinson K, et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016;529:172-7 pubmed 出版商
Hoefflin R, Lahrmann B, Warsow G, Hübschmann D, Spath C, Walter B, et al. Spatial niche formation but not malignant progression is a driving force for intratumoural heterogeneity. Nat Commun. 2016;7:ncomms11845 pubmed 出版商
Takasaki C, Kobayashi M, Ishibashi H, Akashi T, Okubo K. Expression of hypoxia-inducible factor-1α affects tumor proliferation and antiapoptosis in surgically resected lung cancer. Mol Clin Oncol. 2016;5:295-300 pubmed
Narise K, Okuda K, Enomoto Y, Hirayama T, Nagasawa H. Optimization of biguanide derivatives as selective antitumor agents blocking adaptive stress responses in the tumor microenvironment. Drug Des Devel Ther. 2014;8:701-17 pubmed 出版商
Weber D, Tille J, Combescure C, Egger J, Laouiti M, Hammad K, et al. The prognostic value of expression of HIF1α, EGFR and VEGF-A, in localized prostate cancer for intermediate- and high-risk patients treated with radiation therapy with or without androgen deprivation therapy. Radiat Oncol. 2012;7:66 pubmed 出版商
图像
图像 1 :
Novus Biologicals NB100-123L 图像 1
Western Blot: HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488] [NB100-123L] - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts.
图像 2 :
Novus Biologicals NB100-123L 图像 2
Western Blot: HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488] [NB100-123L] - On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB 100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.
图像 3 :
Novus Biologicals NB100-123L 图像 3
Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488] [NB100-123L] - Immunoprecipitation of HIF-1alpha using NB100-123. Heavy and light chains are also detected.
产品信息
目录代码 :
NB100-123L
产品名称 :
Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67) [HiLyte Fluor 488]
标价 :
399美元
产品类型 :
一抗
包装尺寸 :
0.1毫升
别名 :
ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
缓冲液 :
PBS
克隆性 :
单克隆
克隆 :
H1alpha67
共轭标签 :
HiLyte Fluor 488
应用摘要 :
酶联免疫吸附测定, 流式细胞仪, 免疫细胞化学/免疫荧光, 免疫组化, 免疫组化石蜡, 免疫印迹
宿主 :
小鼠
免疫原 :
A fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [UniProt# Q16665].
抗体亚型 :
IgG2b
定位 :
HIF-1 alpha is a nuclear protein that activates gene transcription in response to reduced cellular O2 concentration.
防腐剂 :
0.05% 叠氮化钠
纯度 :
蛋白质A纯化
交叉活性 :
Avian, Bovine, Canine, Ferret, Human, Mouse, Porcine, Primate, Rat, Sheep
储存 :
Store at 4C in the dark.
SwissProt人 :
Q16665
总分子量 :
93
用途 :
This HIF-1 alpha (H1alpha67) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. Chromatin Immunoprecipitation, Gel Super Shift Assays, and ELISA were reported in scientific literature. By Western blot, this antibody recognizes bands at 120kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120kDa representing post-translational modifications. Nuclear extracts are recommended for Western blot.
研究领域 :
Angiogenesis, Cancer, Chromatin Research, Hypoxia, mTOR Pathway, Transcription Factors and Regulators
更多信息或购买 :
公司信息
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
technical@novusbio.com
www.novusbio.com
1-888-506-6887 303-730-1950
公司总部: 美国
Novus Biologicals的任务是开发最先进的新产品及市场行销来加速生物科研的新发现。藉由高效率的组织分工,Novus Biologicals建立了一个将生物学术团体的成果快速商品化的平台。我们的产品除了附有详细的产品资讯外,Novus Biologicals更提供完整的技术支援及服务。利用快速有效的市场通路以便迅速提供所有的科研学者最先进的研究试剂,Novus Biologicals扮演市场上重要的角色。