产品简要
信息索取 :
公司名称 :
Novus Biologicals
其他品牌 :
IMGENEX
产品类型 :
抗体
产品名称 :
HIF-1甲抗体
目录 :
NB100-134
规格 :
0.1毫升
价格 :
349美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
frogs and toads, 人类, 小鼠, 大鼠, , 斑马鱼
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, EMSA, 染色质免疫沉淀 , 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 109
已发表的文献中的应用方法/种属/稀释度参考文献
  • 免疫印迹 (基因敲减 验证); 人类; 图 3
  • 免疫印迹 (基因敲除 验证); 小鼠; 图 3
Mori H, Yao Y, Learman B, Kurozumi K, Ishida J, Ramakrishnan S, et al. Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation, migration and invasion. Sci Rep. 2016;6:21520 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 3a
Yuan X, Qi H, Li X, Wu F, Fang J, Bober E, et al. Disruption of spatiotemporal hypoxic signaling causes congenital heart disease in mice.. J Clin Invest. 2017;127:2235-2248 pubmed 出版商
  • 免疫印迹; 人类; 图 1a
Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell W, Tan A, et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis. 2017;6:e287 pubmed 出版商
  • 染色质免疫沉淀 ; 人类; 图 4f
Salvi V, Vermi W, Gianello V, Lonardi S, Gagliostro V, Naldini A, et al. Dendritic cell-derived VEGF-A plays a role in inflammatory angiogenesis of human secondary lymphoid organs and is driven by the coordinated activation of multiple transcription factors. Oncotarget. 2016;7:39256-39269 pubmed 出版商
  • 免疫沉淀; 人类; 1:200; 图 2f
  • 免疫印迹; 人类; 1:1000; 图 2f
Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, et al. The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer. Nat Cell Biol. 2016;18:213-24 pubmed 出版商
  • 免疫印迹; 小鼠; 1:600
Hung Y, Chang S, Huang C, Yin J, Hwang C, Yang L, et al. Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons. Mol Neurobiol. 2016;53:793-809 pubmed 出版商
  • 免疫印迹; 人类; 图 1
de Jong O, van Balkom B, Gremmels H, Verhaar M. Exosomes from hypoxic endothelial cells have increased collagen crosslinking activity through up-regulation of lysyl oxidase-like 2. J Cell Mol Med. 2016;20:342-50 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠
Semba H, Takeda N, Isagawa T, Sugiura Y, Honda K, Wake M, et al. HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. 2016;7:11635 pubmed 出版商
  • 免疫组化-石蜡切片; 狗; 1:500; 图 5
Spitzbarth I, Lempp C, Kegler K, Ulrich R, Kalkuhl A, Deschl U, et al. Immunohistochemical and transcriptome analyses indicate complex breakdown of axonal transport mechanisms in canine distemper leukoencephalitis. Brain Behav. 2016;6:e00472 pubmed 出版商
  • 染色质免疫沉淀 ; 小鼠; 图 2
Koh H, Chang C, Jeon S, Yoon H, Ahn Y, Kim H, et al. The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia. Nat Commun. 2015;6:6340 pubmed 出版商
  • 免疫组化; 人类; 表 2
  • 免疫印迹; 人类; 表 2
Park I, Chung P, Ahn J. Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation. PLoS ONE. 2015;10:e0122776 pubmed 出版商
  • 免疫印迹; 小鼠
Wang C, Cai Y, Zhang Y, Xiong Z, Li G, Cui L. Local injection of deferoxamine improves neovascularization in ischemic diabetic random flap by increasing HIF-1α and VEGF expression. PLoS ONE. 2014;9:e100818 pubmed 出版商
  • 免疫印迹; 小鼠
Fahrenbach J, Stoller D, Kim G, Aggarwal N, Yerokun B, Earley J, et al. Abcc9 is required for the transition to oxidative metabolism in the newborn heart. FASEB J. 2014;28:2804-15 pubmed 出版商
  • 免疫组化-石蜡切片; 小鼠
Takeda K, Duan L, Takeda H, Fong G. Improved vascular survival and growth in the mouse model of hindlimb ischemia by a remote signaling mechanism. Am J Pathol. 2014;184:686-96 pubmed 出版商
Joyal J, Sun Y, Gantner M, Shao Z, Evans L, Saba N, et al. Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1. Nat Med. 2016;22:439-45 pubmed 出版商
Takahashi N, Davy P, Gardner L, Mathews J, Yamazaki Y, Allsopp R. Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle. PLoS ONE. 2016;11:e0154309 pubmed 出版商
Saeedi B, Kao D, Kitzenberg D, Dobrinskikh E, Schwisow K, Masterson J, et al. HIF-dependent regulation of claudin-1 is central to intestinal epithelial tight junction integrity. Mol Biol Cell. 2015;26:2252-62 pubmed 出版商
Obama T, Takayanagi T, Kobayashi T, Bourne A, Elliott K, Charbonneau M, et al. Vascular induction of a disintegrin and metalloprotease 17 by angiotensin II through hypoxia inducible factor 1α. Am J Hypertens. 2015;28:10-4 pubmed 出版商
Sun K, Chen M, Tu M, Wang I, Chang S, Li C. MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 2015;73:145-53 pubmed 出版商
García L, Leimgruber C, Uribe Echevarría E, Acosta P, Brahamian J, Polack F, et al. Protective phenotypes of club cells and alveolar macrophages are favored as part of endotoxin-mediated prevention of asthma. Exp Biol Med (Maywood). 2015;240:904-16 pubmed 出版商
Khamaisi M, Toukan H, Axelrod J, Rosenberger C, Skarzinski G, Shina A, et al. Endothelin-converting enzyme is a plausible target gene for hypoxia-inducible factor. Kidney Int. 2015;87:761-70 pubmed 出版商
Chen P, Weng J, Hsu P, Shew J, Huang Y, Lee W. NPGPx modulates CPEB2-controlled HIF-1α RNA translation in response to oxidative stress. Nucleic Acids Res. 2015;43:9393-404 pubmed 出版商
Forristal C, Brown A, Helwani F, Winkler I, Nowlan B, Barbier V, et al. Hypoxia inducible factor (HIF)-2α accelerates disease progression in mouse models of leukemia and lymphoma but is not a poor prognosis factor in human AML. Leukemia. 2015;29:2075-85 pubmed 出版商
Wang J, Ding N, Li Y, Cheng H, Wang D, Yang Q, et al. Insulin-like growth factor binding protein 5 (IGFBP5) functions as a tumor suppressor in human melanoma cells. Oncotarget. 2015;6:20636-49 pubmed
Belibi F, Zafar I, Ravichandran K, Segvic A, Jani A, Ljubanovic D, et al. Hypoxia-inducible factor-1α (HIF-1α) and autophagy in polycystic kidney disease (PKD). Am J Physiol Renal Physiol. 2011;300:F1235-43 pubmed 出版商
Ravichandran K, Tyagi A, Deep G, Agarwal C, Agarwal R. Interleukin-1beta-induced iNOS expression in human lung carcinoma A549 cells: involvement of STAT and MAPK pathways. Indian J Exp Biol. 2011;49:840-7 pubmed
Edrey Y, Casper D, Huchon D, Mele J, Gelfond J, Kristan D, et al. Sustained high levels of neuregulin-1 in the longest-lived rodents; a key determinant of rodent longevity. Aging Cell. 2012;11:213-22 pubmed 出版商
Bergeron M, Gidday J, Yu A, Semenza G, Ferriero D, Sharp F. Role of hypoxia-inducible factor-1 in hypoxia-induced ischemic tolerance in neonatal rat brain. Ann Neurol. 2000;48:285-96 pubmed
Copple B, Kaska S, Wentling C. Hypoxia-inducible factor activation in myeloid cells contributes to the development of liver fibrosis in cholestatic mice. J Pharmacol Exp Ther. 2012;341:307-16 pubmed 出版商
Litz J, Krystal G. Imatinib inhibits c-Kit-induced hypoxia-inducible factor-1alpha activity and vascular endothelial growth factor expression in small cell lung cancer cells. Mol Cancer Ther. 2006;5:1415-22 pubmed
Li G, Ci W, Karmakar S, Chen K, Dhar R, Fan Z, et al. SPOP promotes tumorigenesis by acting as a key regulatory hub in kidney cancer. Cancer Cell. 2014;25:455-68 pubmed 出版商
Piantadosi C, Suliman H. Transcriptional Regulation of SDHa flavoprotein by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells. J Biol Chem. 2008;283:10967-77 pubmed 出版商
Robertson E, Wasylyk C, Ye T, Jung A, Wasylyk B. The oncogenic MicroRNA Hsa-miR-155-5p targets the transcription factor ELK3 and links it to the hypoxia response. PLoS ONE. 2014;9:e113050 pubmed 出版商
Chu X, Gao X, Jansson L, Quach M, Skogseid B, Barbu A. Multiple microvascular alterations in pancreatic islets and neuroendocrine tumors of a Men1 mouse model. Am J Pathol. 2013;182:2355-67 pubmed 出版商
Ohtomo S, Nangaku M, Izuhara Y, Takizawa S, Strihou C, Miyata T. Cobalt ameliorates renal injury in an obese, hypertensive type 2 diabetes rat model. Nephrol Dial Transplant. 2008;23:1166-72 pubmed
Neary M, Ng K, Ludtmann M, Hall A, Piotrowska I, Ong S, et al. Hypoxia signaling controls postnatal changes in cardiac mitochondrial morphology and function. J Mol Cell Cardiol. 2014;74:340-52 pubmed 出版商
Erman A, Wabitsch M, Goodyer C. Human growth hormone receptor (GHR) expression in obesity: II. Regulation of the human GHR gene by obesity-related factors. Int J Obes (Lond). 2011;35:1520-9 pubmed 出版商
Morote-García J, Napiwotzky D, Kohler D, Rosenberger P. Endothelial Semaphorin 7A promotes neutrophil migration during hypoxia. Proc Natl Acad Sci U S A. 2012;109:14146-51 pubmed 出版商
Carbajo-Pescador S, Ordonez R, Benet M, Jover R, Garcia-Palomo A, Mauriz J, et al. Inhibition of VEGF expression through blockade of Hif1α and STAT3 signalling mediates the anti-angiogenic effect of melatonin in HepG2 liver cancer cells. Br J Cancer. 2013;109:83-91 pubmed 出版商
Tuomela J, Sandholm J, Karihtala P, Ilvesaro J, Vuopala K, Kauppila J, et al. Low TLR9 expression defines an aggressive subtype of triple-negative breast cancer. Breast Cancer Res Treat. 2012;135:481-93 pubmed 出版商
Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh H, et al. Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements. Genome Biol. 2014;15:R63 pubmed
Li X, Liu X, Xu Y, Liu J, Xie M, Ni W, et al. KLF5 promotes hypoxia-induced survival and inhibits apoptosis in non-small cell lung cancer cells via HIF-1α. Int J Oncol. 2014;45:1507-14 pubmed 出版商
Kanematsu S, Tanimoto K, Suzuki Y, Sugano S. Screening for possible miRNA-mRNA associations in a colon cancer cell line. Gene. 2014;533:520-31 pubmed 出版商
Farrow E, Yu X, Summers L, Davis S, Fleet J, Allen M, et al. Iron deficiency drives an autosomal dominant hypophosphatemic rickets (ADHR) phenotype in fibroblast growth factor-23 (Fgf23) knock-in mice. Proc Natl Acad Sci U S A. 2011;108:E1146-55 pubmed 出版商
Filby C, Hooper S, Wallace M. Partial pulmonary embolization disrupts alveolarization in fetal sheep. Respir Res. 2010;11:42 pubmed 出版商
Glover L, Bowers B, Saeedi B, Ehrentraut S, Campbell E, Bayless A, et al. Control of creatine metabolism by HIF is an endogenous mechanism of barrier regulation in colitis. Proc Natl Acad Sci U S A. 2013;110:19820-5 pubmed 出版商
Zhang H, Bosch-Marce M, Shimoda L, Tan Y, Baek J, Wesley J, et al. Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia. J Biol Chem. 2008;283:10892-903 pubmed 出版商
Vakili H, Jin Y, Cattini P. Negative regulation of human growth hormone gene expression by insulin is dependent on hypoxia-inducible factor binding in primary non-tumor pituitary cells. J Biol Chem. 2012;287:33282-92 pubmed
Mimura I, Nangaku M, Kanki Y, Tsutsumi S, Inoue T, Kohro T, et al. Dynamic change of chromatin conformation in response to hypoxia enhances the expression of GLUT3 (SLC2A3) by cooperative interaction of hypoxia-inducible factor 1 and KDM3A. Mol Cell Biol. 2012;32:3018-32 pubmed 出版商
Enzmann G, Mysiorek C, Gorina R, Cheng Y, Ghavampour S, Hannocks M, et al. The neurovascular unit as a selective barrier to polymorphonuclear granulocyte (PMN) infiltration into the brain after ischemic injury. Acta Neuropathol. 2013;125:395-412 pubmed 出版商
Legendre C, Hori T, Loyer P, Aninat C, Ishida S, Glaise D, et al. Drug-metabolising enzymes are down-regulated by hypoxia in differentiated human hepatoma HepaRG cells: HIF-1alpha involvement in CYP3A4 repression. Eur J Cancer. 2009;45:2882-92 pubmed 出版商
Liu X, Wu X, Cai L, Tang C, Su J. Hypoxic preconditioning of cardiomyocytes and cardioprotection: phophorylation of HIF-1alpha induced by p42/p44 mitogen-activated protein kinases is involved. Pathophysiology. 2003;9:201-205 pubmed
Clambey E, McNamee E, Westrich J, Glover L, Campbell E, Jedlicka P, et al. Hypoxia-inducible factor-1 alpha-dependent induction of FoxP3 drives regulatory T-cell abundance and function during inflammatory hypoxia of the mucosa. Proc Natl Acad Sci U S A. 2012;109:E2784-93 pubmed 出版商
Kim J, Gao P, Liu Y, Semenza G, Dang C. Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1. Mol Cell Biol. 2007;27:7381-93 pubmed
Harris J, Brown G, Jorgensen M, Kaushal S, Ellis E, Grant M, et al. Bone marrow-derived cells home to and regenerate retinal pigment epithelium after injury. Invest Ophthalmol Vis Sci. 2006;47:2108-13 pubmed
Li B, Qiu B, Lee D, Walton Z, Ochocki J, Mathew L, et al. Fructose-1,6-bisphosphatase opposes renal carcinoma progression. Nature. 2014;513:251-5 pubmed 出版商
Xia X, Kung A. Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia. Genome Biol. 2009;10:R113 pubmed 出版商
Guo X, Dason E, Zanon-Moreno V, Jiang Q, Nahirnyj A, Chan D, et al. PGC-1α signaling coordinates susceptibility to metabolic and oxidative injury in the inner retina. Am J Pathol. 2014;184:1017-29 pubmed 出版商
Rennie M, Detmar J, Whiteley K, Jurisicova A, Adamson S, Sled J. Expansion of the fetoplacental vasculature in late gestation is strain dependent in mice. Am J Physiol Heart Circ Physiol. 2012;302:H1261-73 pubmed 出版商
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Yue Y, Zhang P, Liu D, Yang J, Nie C, Yang D. Hypoxia preconditioning enhances the viability of ADSCs to increase the survival rate of ischemic skin flaps in rats. Aesthetic Plast Surg. 2013;37:159-70 pubmed 出版商
Pfäfflin A, Brodbeck K, Heilig C, Haring H, Schleicher E, Weigert C. Increased glucose uptake and metabolism in mesangial cells overexpressing glucose transporter 1 increases interleukin-6 and vascular endothelial growth factor production: role of AP-1 and HIF-1alpha. Cell Physiol Biochem. 2006;18:199-210 pubmed
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Naito Y, Tsujino T, Matsumoto M, Sakoda T, Ohyanagi M, Masuyama T. Adaptive response of the heart to long-term anemia induced by iron deficiency. Am J Physiol Heart Circ Physiol. 2009;296:H585-93 pubmed 出版商
Lin Q, Lee Y, Yun Z. Differentiation arrest by hypoxia. J Biol Chem. 2006;281:30678-83 pubmed
Singh R, Gu M, Agarwal R. Silibinin inhibits colorectal cancer growth by inhibiting tumor cell proliferation and angiogenesis. Cancer Res. 2008;68:2043-50 pubmed 出版商
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Lauzier M, Robitaille G, Chan D, Giaccia A, Richard D. (2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors. Mol Pharmacol. 2008;74:282-8 pubmed 出版商
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Sikder D, Kodadek T. The neurohormone orexin stimulates hypoxia-inducible factor-1 activity. Genes Dev. 2007;21:2995-3005 pubmed
Ganapathy S, Xiao S, Seo S, Lall R, Yang M, Xu T, et al. Low-dose arsenic induces chemotherapy protection via p53/NF-κB-mediated metabolic regulation. Oncogene. 2014;33:1359-66 pubmed 出版商
Oestreich K, Read K, Gilbertson S, Hough K, McDonald P, Krishnamoorthy V, et al. Bcl-6 directly represses the gene program of the glycolysis pathway. Nat Immunol. 2014;15:957-64 pubmed 出版商
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Chiang C, Nangaku M, Tanaka T, Iwawaki T, Inagi R. Endoplasmic reticulum stress signal impairs erythropoietin production: a role for ATF4. Am J Physiol Cell Physiol. 2013;304:C342-53 pubmed 出版商
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Tadokoro H, Umezu T, Ohyashiki K, Hirano T, Ohyashiki J. Exosomes derived from hypoxic leukemia cells enhance tube formation in endothelial cells. J Biol Chem. 2013;288:34343-51 pubmed 出版商
Harvey A, Kind K, Thompson J. Regulation of gene expression in bovine blastocysts in response to oxygen and the iron chelator desferrioxamine. Biol Reprod. 2007;77:93-101 pubmed
Rane S, He M, Sayed D, Vashistha H, Malhotra A, Sadoshima J, et al. Downregulation of miR-199a derepresses hypoxia-inducible factor-1alpha and Sirtuin 1 and recapitulates hypoxia preconditioning in cardiac myocytes. Circ Res. 2009;104:879-86 pubmed 出版商
Hernandez-Luna M, Rocha-Zavaleta L, Vega M, Huerta-Yepez S. Hypoxia inducible factor-1α induces chemoresistance phenotype in non-Hodgkin lymphoma cell line via up-regulation of Bcl-xL. Leuk Lymphoma. 2013;54:1048-55 pubmed 出版商
Eftedal I, Jørgensen A, Røsbjørgen R, Flatberg A, Brubakk A. Early genetic responses in rat vascular tissue after simulated diving. Physiol Genomics. 2012;44:1201-7 pubmed 出版商
Liao D, Corle C, Seagroves T, Johnson R. Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression. Cancer Res. 2007;67:563-72 pubmed
Forristal C, Winkler I, Nowlan B, Barbier V, Walkinshaw G, Levesque J. Pharmacologic stabilization of HIF-1α increases hematopoietic stem cell quiescence in vivo and accelerates blood recovery after severe irradiation. Blood. 2013;121:759-69 pubmed 出版商
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Prasad P, Gordijo C, Abbasi A, Maeda A, Ip A, Rauth A, et al. Multifunctional albumin-MnO₂ nanoparticles modulate solid tumor microenvironment by attenuating hypoxia, acidosis, vascular endothelial growth factor and enhance radiation response. ACS Nano. 2014;8:3202-12 pubmed 出版商
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Sena J, Wang L, Hu C. BRG1 and BRM chromatin-remodeling complexes regulate the hypoxia response by acting as coactivators for a subset of hypoxia-inducible transcription factor target genes. Mol Cell Biol. 2013;33:3849-63 pubmed 出版商
Nikinmaa M, Leveelahti L, Dahl E, Rissanen E, Rytkönen K, Laurila A. Population origin, development and temperature of development affect the amounts of HSP70, HSP90 and the putative hypoxia-inducible factor in the tadpoles of the common frog Rana temporaria. J Exp Biol. 2008;211:1999-2004 pubmed 出版商
Bansal A, Harris R, DeGrado T. Choline phosphorylation and regulation of transcription of choline kinase α in hypoxia. J Lipid Res. 2012;53:149-57 pubmed 出版商
Aguado-Fraile E, Ramos E, Sáenz-Morales D, Conde E, Blanco-Sánchez I, Stamatakis K, et al. miR-127 protects proximal tubule cells against ischemia/reperfusion: identification of kinesin family member 3B as miR-127 target. PLoS ONE. 2012;7:e44305 pubmed 出版商
Land S, Wilson S. Redox regulation of lung development and perinatal lung epithelial function. Antioxid Redox Signal. 2005;7:92-107 pubmed
Dong Y, Ito T, Velayo C, Sato T, Iida K, Endo M, et al. Intrauterine ischemic reperfusion switches the fetal transcriptional pattern from HIF-1α- to P53-dependent regulation in the murine brain. PLoS ONE. 2014;9:e110577 pubmed 出版商
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Regan Anderson T, Peacock D, Daniel A, Hubbard G, Lofgren K, Girard B, et al. Breast tumor kinase (Brk/PTK6) is a mediator of hypoxia-associated breast cancer progression. Cancer Res. 2013;73:5810-20 pubmed 出版商
Tanimoto K, Tsuchihara K, Kanai A, Arauchi T, Esumi H, Suzuki Y, et al. Genome-wide identification and annotation of HIF-1α binding sites in two cell lines using massively parallel sequencing. Hugo J. 2010;4:35-48 pubmed 出版商
Wang Y, Huang J, Li M, Cavenee W, Mitchell P, Zhou X, et al. MicroRNA-138 modulates DNA damage response by repressing histone H2AX expression. Mol Cancer Res. 2011;9:1100-11 pubmed 出版商
Botusan I, Sunkari V, Savu O, Catrina A, Grunler J, Lindberg S, et al. Stabilization of HIF-1alpha is critical to improve wound healing in diabetic mice. Proc Natl Acad Sci U S A. 2008;105:19426-31 pubmed 出版商
Zecchini V, Madhu B, Russell R, Pértega-Gomes N, Warren A, Gaude E, et al. Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer. EMBO J. 2014;33:1365-82 pubmed 出版商
图像
图像 1 :
Novus Biologicals NB100-134 图像 1
Simple Western: HIF-1 alpha Antibody [NB100-134] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.2 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
图像 2 :
Novus Biologicals NB100-134 图像 2
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF1 alpha antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).
图像 3 :
Novus Biologicals NB100-134 图像 3
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Staining in the canine CL on days 10 to 70 after ovulation. PC = positive control (human placenta). NC = negative control. Image from confirmed customer review.
产品信息
目录代码 :
NB100-134
产品名称 :
HIF-1甲抗体
标价 :
349美元
产品类型 :
一抗
包装尺寸 :
0.1毫升
别名 :
ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
缓冲液 :
PBS
克隆性 :
多克隆
浓度 :
1.0
应用摘要 :
Chromatin Immunoprecipitation, Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
宿主 :
免疫原 :
A fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [UniProt Q16665].
定位 :
Nucleus
防腐剂 :
0.05% 叠氮化钠
纯度 :
免疫原亲和纯化
交叉活性 :
狗, 豚鼠, 人类, 小鼠, 灵长类动物, 大鼠, 蛙, 斑马鱼
储存 :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
SwissProt人 :
Q16665
总分子量 :
93
用途 :
This HIF-1 alpha antibody is useful for Chromatin Immunoprecipitation (PMID 22891341), Immunohistochemistry, Western Blot, Gel Shift Assays, Immunocytochemistry/Immunfluorescence (PMID: 21270095; 21270095), and Immunoprecipitation. In Western blot a band is observed ~120 kDa. IHC applications have been mixed on mouse. For ChIP, nuclear extracts are recommended. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
研究领域 :
Angiogenesis, Autophagy, Cancer, Cellular Markers, Chromatin Research, HIF Target Genes, Hypoxia, Hypoxia Signaling, mTOR Pathway, Transcription Factors and Regulators
更多信息或购买 :
公司信息
Novus Biologicals
8100 Southpark Way, A-8
Littleton, CO 80120
technical@novusbio.com
www.novusbio.com
1-888-506-6887 303-730-1950
公司总部: 美国
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