产品简要
公司名称 :
Novus Biologicals
其他品牌 :
IMGENEX
产品类型 :
抗体
产品名称 :
RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free
目录 :
NB200-598
规格 :
0.1 ml (also 0.025 ml)
价格 :
399美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
4H8
反应物种 :
人类, 小鼠, brewer's yeast
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 染色质免疫沉淀
更多信息或购买 :
文章摘录数: 30
出版应用/物种/样本/稀释参考文献
  • 染色质免疫沉淀 ; 人类; 图 11
Luo W, Chen I, Chen Y, Alkam D, Wang Y, Semenza G. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia. Oncotarget. 2016;7:6379-97 pubmed 出版商
  • 免疫细胞化学; 小鼠
Chan E, Teng G, Corbett E, Choudhury K, Bassing C, Schatz D, et al. Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2. Proc Natl Acad Sci U S A. 2013;110:E4628-37 pubmed 出版商
  • 染色质免疫沉淀 ; brewer's yeast
Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T. Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3. Sci Rep. 2013;3:2186 pubmed 出版商
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Cheng J, Torkamani A, Peng Y, Jones T, Lerner R. Plasma membrane associated transcription of cytoplasmic DNA. Proc Natl Acad Sci U S A. 2012;109:10827-31 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Walsh H, Shupnik M. Proteasome regulation of dynamic transcription factor occupancy on the GnRH-stimulated luteinizing hormone beta-subunit promoter. Mol Endocrinol. 2009;23:237-50 pubmed 出版商
  • 免疫沉淀; brewer's yeast
  • 免疫印迹; brewer's yeast
Reid J, Svejstrup J. DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro. J Biol Chem. 2004;279:29875-8 pubmed
Kim Y, Qi M, Dong X, Seok S, Sun H, Kemper B, et al. Transgenic mice lacking FGF15/19-SHP phosphorylation display altered bile acids and gut bacteria, promoting nonalcoholic fatty liver disease. J Biol Chem. 2023;299:104946 pubmed 出版商
Li F, Negi V, Yang P, Lee J, Ma K, Moulik M, et al. TEAD1 regulates cell proliferation through a pocket-independent transcription repression mechanism. Nucleic Acids Res. 2022;50:12723-12738 pubmed 出版商
Hensel A, Stahl P, Moews L, K xf6 nig L, Patwardhan R, H xf6 ing A, et al. The Taspase1/Myosin1f-axis regulates filopodia dynamics. iScience. 2022;25:104355 pubmed 出版商
Zhang L, Qu J, Qi Y, Duan Y, Huang Y, Zhou Z, et al. EZH2 engages TGFβ signaling to promote breast cancer bone metastasis via integrin β1-FAK activation. Nat Commun. 2022;13:2543 pubmed 出版商
Appanah R, Lones E, Aiello U, Libri D, De Piccoli G. Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability. Cell Rep. 2020;30:2094-2105.e9 pubmed 出版商
Taïb B, Aboussalah A, Moniruzzaman M, Chen S, Haughey N, Kim S, et al. Lipid accumulation and oxidation in glioblastoma multiforme. Sci Rep. 2019;9:19593 pubmed 出版商
Helsley R, Varadharajan V, Brown A, Gromovsky A, Schugar R, Ramachandiran I, et al. Obesity-linked suppression of membrane-bound O-acyltransferase 7 (MBOAT7) drives non-alcoholic fatty liver disease. elife. 2019;8: pubmed 出版商
Eng J, Thibault G, Luoh S, Gray J, Chang Y, Chin K. Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis. Methods Mol Biol. 2020;2055:521-562 pubmed 出版商
Asimakopoulou A, Vucur M, Luedde T, Schneiders S, Kalampoka S, Weiss T, et al. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma. Cancers (Basel). 2019;11: pubmed 出版商
Farmer B, Kluemper J, Johnson L. Apolipoprotein E4 Alters Astrocyte Fatty Acid Metabolism and Lipid Droplet Formation. Cells. 2019;8: pubmed 出版商
Chali F, Milior G, Marty S, Morin Brureau M, Le Duigou C, Savary E, et al. Lipid markers and related transcripts during excitotoxic neurodegeneration in kainate-treated mice. Eur J Neurosci. 2019;50:1759-1778 pubmed 出版商
Zhang P, Csaki L, Ronquillo E, Baufeld L, Lin J, Gutiérrez A, et al. Lipin 2/3 phosphatidic acid phosphatases maintain phospholipid homeostasis to regulate chylomicron synthesis. J Clin Invest. 2019;129:281-295 pubmed 出版商
Larsen S, Vigelsø A, Dandanell S, Prats C, Dela F, Helge J. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. J Diabetes Res. 2018;2018:9257874 pubmed 出版商
Li Z, Rasmussen M, Li J, Henríquez Olguín C, Knudsen J, Madsen A, et al. Periodized low protein-high carbohydrate diet confers potent, but transient, metabolic improvements. Mol Metab. 2018;17:112-121 pubmed 出版商
Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed 出版商
Madushani Herath K, Cho J, Kim A, Eom T, Kim J, Kim J, et al. Phenolic acid and flavonoid-rich fraction of Sasa quelpaertensis Nakai leaves prevent alcohol induced fatty liver through AMPK activation. J Ethnopharmacol. 2018;224:335-348 pubmed 出版商
Blossom S, Fernandes L, Bai S, Khare S, Gokulan K, Yuan Y, et al. Opposing Actions of Developmental Trichloroethylene and High-Fat Diet Coexposure on Markers of Lipogenesis and Inflammation in Autoimmune-Prone Mice. Toxicol Sci. 2018;164:313-327 pubmed 出版商
Clemente C, Rius C, Alonso Herranz L, Martín Alonso M, Pollán A, Camafeita E, et al. MT4-MMP deficiency increases patrolling monocyte recruitment to early lesions and accelerates atherosclerosis. Nat Commun. 2018;9:910 pubmed 出版商
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Kim S, Lee J, Kim T, Kim Y, Chung K. Fat Graft Survival After Recipient Site Pretreatment With Fractional Carbon Dioxide Laser. Ann Plast Surg. 2017;79:552-557 pubmed 出版商
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Nishikawa K, Iwaya K, Kinoshita M, Fujiwara Y, Akao M, Sonoda M, et al. Resveratrol increases CD68⁺ Kupffer cells colocalized with adipose differentiation-related protein and ameliorates high-fat-diet-induced fatty liver in mice. Mol Nutr Food Res. 2015;59:1155-70 pubmed 出版商
产品信息
品牌 :
Novus Biologicals, a Bio-Techne Brand
catalog number base :
NB200-598
SKU号 :
NB200-598
产品名称 :
RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free
描述 :
The RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free from Novus is a mouse monoclonal antibody to RNA Polymerase II/POLR2A. This antibody reacts with human, mouse, yeast, primate (negative). The RNA Polymerase II/POLR2A Antibody (4H8) - Azide and BSA Free has been validated for the following applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP), CyTOF-ready.
靶标 :
RNA聚合酶II/POLR2A
类别 :
一抗
单位尺寸 :
0.1 ml (also 0.025 ml)
缓冲液 :
PBS
克隆性 :
单克隆
克隆 :
4H8
浓度 :
1毫克/毫升
共轭标签 :
未共轭
宿主 :
小鼠
免疫原 :
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
抗体亚型 :
IgG1
纯度 :
蛋白质G纯化
物种 :
Human, Mouse, Yeast, Primate (Negative)
特异性 :
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein
理论上的分子量 :
217 kDa
基因符号 :
POLR2A
accessionNumbers :
P24928
应用 :
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP), CyTOF-ready
美元 :
399美元
别名 :
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
储存 :
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
更多信息或购买 :
公司信息
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
technical@novusbio.com
https://www.novusbio.com
1-888-506-6887 303-730-1950
公司总部: 美国
Novus Biologicals的任务是开发最先进的新产品及市场行销来加速生物科研的新发现。藉由高效率的组织分工,Novus Biologicals建立了一个将生物学术团体的成果快速商品化的平台。我们的产品除了附有详细的产品资讯外,Novus Biologicals更提供完整的技术支援及服务。利用快速有效的市场通路以便迅速提供所有的科研学者最先进的研究试剂,Novus Biologicals扮演市场上重要的角色。