产品简要
公司名称 :
Novus Biologicals
其他品牌 :
IMGENEX
产品类型 :
抗体
产品名称 :
ER乙/NR3A2抗体
目录 :
NB300-747
规格 :
50 ug (also 25 ug)
价格 :
329美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
图像
图像 1 :
Novus Biologicals NB300-747 图像 1
Western Blot: ER beta/NR3A2 Antibody [NB300-747] - Analysis of ER beta was performed by loading the indicated amounts of recombinant full-length human ER alpha and ER beta proteins, and 10ul of PageRuler Prestained Protein Ladder per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Blotter, and blocked with 5% milk in TBST for at least 1 hour at room temp. ER beta was detected at ~54kD (left panel) using an ER beta polyclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Pico. The blot was then stripped with Restore Plus Western blot stripping buffer for at least 15 min at room temp, re-blocked with 5% milk in TBST for at least 1 hour at room temp, and re-probed with an ER alpha polyclonal antibody at a dilution of 1:2000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Pico. The presence of faint ER-beta bands on the blot probed.
产品信息
品牌 :
Novus
master code :
NB300-747
SKU号 :
NB300-747
产品名称 :
ER乙/NR3A2抗体
描述 :
The ER beta/NR3A2 Antibody from Novus is a rabbit polyclonal antibody to ER beta/NR3A2. This antibody reacts with human, rat. The ER beta/NR3A2 Antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunofluorescence.
靶标 :
ER乙/NR3A2
类别 :
一抗
sizes available :
50 ug (also 25 ug)
缓冲液 :
PBS with 1 mg/ml BSA
克隆性 :
多克隆
浓度 :
1毫克/毫升
共轭标签 :
未共轭
宿主 :
免疫原 :
合成多肽对应于氨基酸残基C(459)S P A E D S K S K E G S Q N P Q S Q(477)人的ER乙
抗体亚型 :
IgG
纯度 :
免疫原亲和纯化
物种 :
人类, 大鼠
特异性 :
Detects estrogen receptor (ER) beta from human and rat tissues
基因符号 :
ESR2
images :
https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/ER-beta-NR3A2-Antibody-Western-Blot-NB300-747-img0004.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/ER-beta-NR3A2-Antibody-Western-Blot-NB300-747-img0004.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/ER-beta-NR3A2-Antibody-Western-Blot-NB300-747-img0004.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/ER-beta-NR3A2-Antibody-Immunohistochemistry-Frozen-NB300-747-img0003.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/ER-beta-NR3A2-Antibody-Immunohistochemistry-Frozen-NB300-747-img0003.jpg, https://aeroglobalimagesprod.blob.core.windows.net/images/datasheets/ER-beta-NR3A2-Antibody-Immunohistochemistry-Frozen-NB300-747-img0003.jpg
accessionNumbers :
Q92731
应用 :
免疫印迹, 免疫组化, 免疫组化石蜡, 免疫组化冷冻, 免疫荧光
美元 :
329美元
别名 :
Erb, ER-beta, ESR-BETA, ESTRB, estrogen receptor 2 (ER beta), estrogen receptor beta, estrogen receptor beta 4, NR3A2ESRB, Nuclear receptor subfamily 3 group A member 2
储存 :
存放在-20C。 避免冻融循环
更多信息或购买 :
公司信息
Novus Biologicals
10771 E Easter Ave
Centennial, CO 80112
technical@novusbio.com
https://www.novusbio.com
1-888-506-6887 303-730-1950
公司总部: 美国
Novus Biologicals的任务是开发最先进的新产品及市场行销来加速生物科研的新发现。藉由高效率的组织分工,Novus Biologicals建立了一个将生物学术团体的成果快速商品化的平台。我们的产品除了附有详细的产品资讯外,Novus Biologicals更提供完整的技术支援及服务。利用快速有效的市场通路以便迅速提供所有的科研学者最先进的研究试剂,Novus Biologicals扮演市场上重要的角色。