抗体

小鼠单克隆抗体P16(小鼠和人类)

20130

100µl

374.00美元

单克隆

小鼠

nonconjugated

1E12E10

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化

小鼠单克隆抗体P16(小鼠和人类)

人类, 大鼠, 小鼠

1E12E10;

小鼠IgG1

100µl

4?;-20?为长期储存

The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.

Purified recombinant fragment of P16 expressed in E. Coli.

腹水含0.03% 叠氮化钠

ELISA: 1/10000;WB: 1/500 - 1/2000;IHC: 1/200 - 1/1000

1. Hunter, T. 1993. Cell 75: 839-841. ; 2. Sherr, C.J. 1993. Cell 73: 1059-1065. ; 3. El-Deiry, W.S., et al. 1993. Cell 75: 817-825. ;

374.00美元

单克隆抗体

P16