湖南远泰生物技术有限公司

抗体

小鼠单克隆抗体BRAF

20323

100μl

308.00美元

单克隆

小鼠

1H12;

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学

20323

小鼠单克隆抗体BRAF

人类, 小鼠

1H12;

小鼠IgG1

100μl

4℃;-20℃为长期储存

BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

纯化重组片段对应于人BRAF在大肠杆菌中表达。;

腹水含0.03% 叠氮化钠

ELISA: 1/10000;WB: 1/500 - 1/2000;IHC: 1/200 - 1/1000;ICC: 1/200 - 1/1000

1. J Clin Oncol. 2008 Dec 10;26(35):5705-12. ; 2. Endocr Relat Cancer. 2006 Mar;13(1):257-69.

308.00美元

单克隆抗体

87kDa

BRAF1;RAFB1;B-RAF1;FLJ95109