抗体

小鼠单克隆抗体p44/42 MAPK(Erk1/2)

30014

100μl

308.00美元

单克隆

小鼠

nonconjugated

#3F8

人类, 小鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪

小鼠单克隆抗体p44/42 MAPK(Erk1/2)

人类;小鼠

3F8;

小鼠IgG2b

100μl

4℃;-20℃为长期储存

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

纯化重组片段对应于人MAPK在大肠杆菌中表达

腹水含0.03% 叠氮化钠

ELISA: 1/10000;WB: 1/500 - 1/2000;IHC: 1/200 - 1/1000;FCM: 1/200 - 1/400

1. FEBS Lett. 1992 Jun 15;304(2-3):170-8. ; 2. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5361-5. ; 3. J Biol Chem. 2009 Nov 27;284(48):33456-65. ; 4. BMC Biol. 2009 Oct 27;7:70. ;

308.00美元

单克隆抗体

42kDa

ERK;p38;p40;p41;ERK2;ERT1;MAPK2;PRKM1;PRKM2;丝裂原活化蛋白激酶P42;p41mapk;MAPK1