抗体

小鼠单克隆抗体IHOG

30057

100μl

308.00美元

单克隆

小鼠

nonconjugated

3G8

免疫印迹, 酶联免疫吸附测定

小鼠单克隆抗体IHOG

黑腹果蝇

3G8;

小鼠IgG1

100μl

4℃;-20℃为长期储存

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

纯化重组片段对应于人IHOG在大肠杆菌中表达

腹水含0.03% 叠氮化钠

ELISA: 1/10000;WB: 1/500 - 1/2000

1. Cell. 2006 Apr 21;125(2):343-57. ; 2. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17208-13.

308.00美元

单克隆抗体

98kDa

CG9211; CT26314; Dmel CG9211; ihog; Ihog