抗体

PKR抗体

3949

0.02毫克, 0.1毫克

79, 315美元

多克隆

兔

nonconjugated

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, proximity ligation assay

PKR抗体

兔

多克隆

未共轭

一抗

0.02毫克, 0.1毫克

79, 315美元

IgG

人类, 小鼠, 大鼠

PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

PKR antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PKR.

PKR Antibody is affinity chromatography purified via peptide column.

PKR Antibody is supplied in PBS containing 0.02% sodium azide.

1毫克/毫升

E, 免疫印迹, 免疫组化

PKR antibody can be used for detection of PKR by Western blot at 1 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 5 g/mL.

PKR antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Meurs E, Chong K, Galabru J, et al. Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon. Cell 1990; 62:379-90.

Saelens X, Kalai M, and Vandenabeele P. Translation inhibition in apoptosis: Caspase-dependent PKR activation and eIF2-alpha phosphorylation. J. Biol. Chem. 2001; 276:41620-8.

Gil J and Esteban M. Induction of apoptosis by the dsRNA-dependent protein kinase (PKR): Mechanism of action. Apoptosis 2000; 5:107-14.

Gil J, Garcia MA, and Esteban M. Caspase 9 activation by the dsRNA-dependent protein kinase, PKR: Molecular mechanism and relevance. FEBS Lett. 2002; 19:3665-74.