抗体

ORAI1抗体[6D11A11]

PM-5207

0.02毫克, 0.1毫克

79, 315美元

单克隆

小鼠

nonconjugated

6D11A11

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学

ORAI1抗体[6D11A11]

小鼠

单克隆

未共轭

一抗

0.02毫克, 0.1毫克

79, 315美元

6D11A11

IgG2b

人类, 小鼠, 大鼠

ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

ORAI1抗体[6D11A11]: IMD9, ORAT1, CRACM1, TMEM142A

Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

ORAI1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

1毫克/毫升

E, 免疫印迹, 免疫组化, IF

ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

ORAI1 monoclonal antibody can be stored at -20 C, stable for one year.

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.