产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗蛋白质二硫键异构酶 (PDI)抗体
目录 :
11081
规格 :
100微升
价格 :
442美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, 犬, 牛
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 11081 图像 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (11081). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (11081) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite.
图像 2 :
QED Bioscience 11081 图像 2
Western blot analysis of Rat tissue mix showing detection of PDI protein using Rabbit Anti-PDI Polyclonal Antibody (11081). Load: 15 µgprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (11081) at 1:4000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
图像 3 :
QED Bioscience 11081 图像 3
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PDI Polyclonal Antibody (11081). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PDI Polyclonal Antibody (11081) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PDI Antibody. (C) Composite.
产品信息
目录号 :
11081
产品名称 :
抗蛋白质二硫键异构酶 (PDI)抗体
NAME+SKU :
抗蛋白质二硫键异构酶 (PDI)抗体(11081)
产品_TYPE :
抗体
描述 :
抗PDI兔多克隆抗体
SPECIFICITY SHORT :
PDI
物种活性 :
Human, monkey, mouse, rat, hamster, bovine, ovine, porcine, canine, guinea pig, Xenopus
靶标名 :
蛋白质二硫键异构酶 (PDI)
UniProt数据库 :
P04785
LOCATION :
Endoplasmic reticulum , Endoplasmic reticulum lumen , Melanosome , Cell membrane
蛋白名 :
蛋白质二硫键异构酶
别称 :
PDI, EC 5.3.4.1, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta
基因名称 :
P4hb
登录号 :
NP_001099245.2
克隆性 :
多克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微升
标价规格1 :
442美元
DISTIRBUTOR价格 :
309.4
SKU号规格1 :
11081
应用 :
免疫印迹, 免疫组化, 免疫沉淀
应用INSTRUCTIONS :
Western blot: Use at 1:4,000 dilution. A band of approx. 58 kD is detected. Immunocytochemistry/Immunohisto- chemistry: Use at 2-5 ug/ml. Immunoprecipitation: Use at 10 ug/ml.
宿主 :
兔
格式 :
血清
免疫原 :
Synthetic peptide derived from rat PDI conjugated to KLH.
研究领域 :
神经科学
配方 :
Whole Antiserum
缓冲液 :
Whole Antiserum
靶标背景 :
A microsomal enzyme known as Protein Disulfide Isomerase (PDI) is involved in the formation of disulfide bonds in many extracellular proteins through its oxidase and isomerase activities. It is also involved in the reduction of disulfide bonds in proteins. PDI is located primarily in the endoplasmic reticulum (ER) but may also be found in the cytosol. PDI can reside in the ER due to the highly conserved KDEL sequence at its carboxy terminus which serves as an ER-retention signal.
FUNCTION :
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration. {UniProtKB:P07237}.
稀释INSTRUCTIONS :
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
特异性 :
This antibody recognizes human, monkey, mouse, rat, hamster, bovine, ovine, porcine, canine, guinea pig, and Xenopus PDI.
图像Name_1 :
qed-bioscience-anti-protein-disulfide-isomerase-pdi-antibody-11081-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-protein-disulfide-isomerase-pdi-antibody-11081-2.png
应用2 :
免疫印迹
图像Name_3 :
qed-bioscience-anti-protein-disulfide-isomerase-pdi-antibody-11081-3.png
应用3 :
免疫细胞化学
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国