抗体

Anti-Smac (mouse CT) Antibody

1411

100微克

455美元

多克隆

domestic rabbit

未共轭

人类, 小鼠

Anti-Smac (mouse CT) Antibody

Anti-Smac (mouse CT) Antibody (1411)

抗体

Anti-mSmac (CT) Rabbit Polyclonal Antibody

mSmac (CT)

人类, 小鼠, 大鼠

Smac (mouse CT)

Q9JIQ3

线粒体

Diablo IAP-binding mitochondrial protein

Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac

Diablo

NP_075721

多克隆

液体

Lot Specific

100微克

455美元

318.5

1411

免疫印迹, 免疫组化

Immunoblotting: use at 1ug/ml. Positive control: Mouse heart tissue lysate. Immunohistochemistry: use at 2ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

兔

纯化

经免疫亲和层析纯化

Synthetic peptide corresponding to aa 222-237 of mouse Smac (accession no. AF203914).

凋亡

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

Inhibitors of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases has been identified and designated Smac/DIABLO. Smac is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf- 1/caspase-9 pathway. The N-terminal amino acids of Smac are required for binding to IAPs and for activation of caspases. Smac is expressed in a variety of human and mouse tissues.

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity). {PubMed:10929712}.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze-thaw cycles.

This antibody recognizes human, mouse, and rat Smac (25kDa).

qed-bioscience-anti-smac-mouse-ct-antibody-1411-1.jpg

免疫印迹

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免疫印迹

qed-bioscience-anti-smac-mouse-ct-antibody-1411-3.gif

免疫印迹

qed-bioscience-anti-smac-mouse-ct-antibody-1411-4.jpg

Immunofluorescence Validation of Smac in Mouse Spleen Cells . Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Spleen Cells labeling Smac with 1411 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

IF

qed-bioscience-anti-smac-mouse-ct-antibody-1411-5.jpg

Immunohistochemistry Validation of Smac in Mouse Spleen Tissue . Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-Smac antibody (1411) at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

免疫组化

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KO Validation in Mouse Fibroblasts and Myoblasts (Ho et al., 2416) . The indicated MEFs or MEMs were exposed to 2 uM STS for 4 h and analyzed by Western blot. Accumulation of Smac/Diablo in mitochondrion-depleted cytosol fractions fromSTS-treated Apaf-1 KO cells were detected by anti-smac antibodies. Smac expression was not detected in smac KO mice.

免疫印迹

qed-bioscience-anti-smac-mouse-ct-antibody-1411-7.gif

Immunohistochemistry Validation of Smac in Human gastric carcinoma (Kim et al., 2011) . Smac was highly expressed in gastric mucosa of patients with gastric carcinoma.

免疫组化

qed-bioscience-anti-smac-mouse-ct-antibody-1411-8.gif

Immunofluorescence Analysis of Smac in NB4-LR1 Cells (Saumet et al., 2005) . NB4-LR1 cells were either treated with ATRA(1 uM) for 3 days without or with the T3C1 recombinant fragment (3 uM) or treated with staurosporine (STP; 5 uM ) for 3.5 hours. STP, but not ATRA or AYRA/T3C1 induced the release of smac.

IF

qed-bioscience-anti-smac-mouse-ct-antibody-1411-9.gif

Induced Expression Validation in Rat Liver (Genestier et al., 2005) . Mitochondria from rat liver were treated with increasing concentrations of rPVL (A), rLukS (B), or Bax alpha (C) for 1 hour at 30 C. rPVL induces the release of the apoptogenic proteins cytochrome c and Smac/DIABLO from isolated mitochondria.

免疫印迹

qed-bioscience-anti-smac-mouse-ct-antibody-1411-10.gif

Overxpression Validation in HEK293T Cells (Flygare et al., 2012) . HEK293T cells were transiently transfected with Smac and Myc-tagged cIAP1, cIAP2, ML-IAP, or empty vector. Cells were lysed, and lysates were incubated with the indicated concentrations of 1 and immunoprecipitated with anti-Myc antibody (left panels). Samples were then immunoblotted with anti-Smac and anti-Myc antibodies. Whole-cell lysates are shown in the right panel.

免疫沉淀