产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗IKKalpha抗体
目录 :
2115
规格 :
100微克
价格 :
455美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
更多信息或购买 :
图像
图像 1 :
QED Bioscience 2115 图像 1
Western blot analysis of IKK alpha in HeLa cell lysate with IKK alpha antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
图像 2 :
QED Bioscience 2115 图像 2
Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 ug/mL.
图像 3 :
QED Bioscience 2115 图像 3
Immunofluorescence of IKKa in HeLa cells with IKKa antibody at 2 ug/mL.
产品信息
目录号 :
2115
产品名称 :
抗IKKalpha抗体
NAME+SKU :
抗IKKalpha抗体(2115)
产品_TYPE :
抗体
描述 :
抗IKK甲兔多克隆抗体
SPECIFICITY SHORT :
IKK甲
物种活性 :
人类
靶标名 :
IKKalpha
UniProt数据库 :
O15111
LOCATION :
Cytoplasm , Nucleus
蛋白名 :
Inhibitor of nuclear factor kappa-B kinase subunit alpha
别称 :
I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, EC 2.7.11.10, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16
基因名称 :
CHUK
登录号 :
NP_001269
克隆性 :
多克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
455美元
DISTIRBUTOR价格 :
318.5
SKU号规格1 :
2115
应用 :
免疫印迹
应用INSTRUCTIONS :
Immunoblotting: use at 1:500-1:1,000 dilution. Positive control: Whole cell lysate from HeLa or Jurkat cells.
宿主 :
格式 :
纯化
纯化 :
经免疫亲和层析纯化
免疫原 :
Peptide corresponding to aa 699-715 of human IKKalpha which differs from the corresponding murine sequence by one amino acid.
研究领域 :
神经科学
配方 :
PBS, pH值7.4
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
靶标背景 :
Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-kappaB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFalpha, and LPS. NF-kappaB is associated with IkappaB in cytoplasm, which inhibits NF-kappaB activity. IkappaB kinase (IKK), which phosphorylates IkappaB and mediates IkappaB degradation and NF-kappaB activation, was recently identified. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKalpha and IKKbeta interact with each other, and both are essential for NF-B activation. IKKalpha specifically phosphorylates IkappaB-alpha and is expressed in a variety of human tissues.
FUNCTION :
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (PubMed:9244310, PubMed:9252186, PubMed:9346484, PubMed:18626576). Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues (PubMed:9244310, PubMed:9252186, PubMed:9346484, PubMed:18626576). These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome (PubMed:9244310, PubMed:9252186, PubMed:9346484, PubMed:18626576). In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis (PubMed:9244310, PubMed:9252186, PubMed:9346484, PubMed:18626576). Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11) (PubMed:21765415). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes (PubMed:20501937). In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities (PubMed:17434128). Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP (PubMed:12789342). Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor (PubMed:15084260). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates AMBRA1 following mitophagy induction, promoting AMBRA1 interaction with ATG8 family proteins and its mitophagic activity (PubMed:30217973). {UniProtKB:Q60680, PubMed:12789342, PubMed:15084260, PubMed:17434128, PubMed:20434986, PubMed:20501937, PubMed:21765415, PubMed:30217973, PubMed:9244310, PubMed:9252186, PubMed:9346484, PubMed:18626576}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze-thaw cycles.
特异性 :
This antibody recognizes human IKKalpha (85 kD). No cross-reactivity with IKKbeta or IKKgamma.
图像Name_1 :
qed-bioscience-anti-ikkalpha-antibody-2115-1.gif
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-anti-ikkalpha-antibody-2115-2.jpg
应用2 :
免疫细胞化学
图像Name_3 :
qed-bioscience-anti-ikkalpha-antibody-2115-3.jpg
应用3 :
IF
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国