产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗IKKbeta抗体
目录 :
2121
规格 :
100微克
价格 :
455美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
更多信息或购买 :
图像
图像 1 :
QED Bioscience 2121 图像 1
Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 ug/mL.
图像 2 :
QED Bioscience 2121 图像 2
Immunofluorescence of IKK beta in Hela cells with IKK beta antibody at 10 ug/mL.
产品信息
目录号 :
2121
产品名称 :
抗IKKbeta抗体
NAME+SKU :
抗IKKbeta抗体(2121)
产品_TYPE :
抗体
描述 :
Anti-Ikk beta Rabbit Polyclonal Antibody
SPECIFICITY SHORT :
Ikk beta
物种活性 :
人类
靶标名 :
IKKbeta
UniProt数据库 :
O14920
LOCATION :
Cytoplasm , Nucleus , Membrane raft
蛋白名 :
Inhibitor of nuclear factor kappa-B kinase subunit beta
别称 :
I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, EC 2.7.11.10, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, Serine/threonine protein kinase IKBKB, EC 2.7.11.1
基因名称 :
IKBKB
登录号 :
NP_001547
克隆性 :
多克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
455美元
DISTIRBUTOR价格 :
318.5
SKU号规格1 :
2121
应用 :
免疫印迹
应用INSTRUCTIONS :
Immunoblotting: use at 1:500-1:1,000 dilution. Positive control: Whole cell lysate from Jurkat cells.
宿主 :
格式 :
纯化
纯化 :
经免疫亲和层析纯化
免疫原 :
Peptide corresponding to aa 662-680 of human IKKbeta which differs from the corresponding murine sequence by one amino acid.
研究领域 :
神经科学
配方 :
PBS, pH值7.4
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
靶标背景 :
Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-kappaB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFalpha, and LPS. NF-kappaB is associated with IkappaB in cytoplasm, which inhibits NF-kappaB activity. IkappaB kinase (IKK), which phosphorylates IkappaB and mediates IkappaB degradation and NF-kappaB activation, was recently identified. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKalpha and IKKbeta interact with each other, and both are essential for NF-B activation. IKKbeta phosphorylates both IkappaB-alpha and IkappaB-beta and is expressed in a variety of human tissues.
FUNCTION :
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses (PubMed:30337470). Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE (PubMed:11297557, PubMed:20410276). IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor (PubMed:15084260). Also phosphorylates other substrates including NCOA3, BCL10 and IRS1 (PubMed:17213322). Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation (PubMed:11297557). Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus (PubMed:25326418). {UniProtKB:O88351, PubMed:11297557, PubMed:15084260, PubMed:17213322, PubMed:19716809, PubMed:20410276, PubMed:20434986, PubMed:20797629, PubMed:21138416, PubMed:25326418, PubMed:30337470}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze- thaw cycles.
特异性 :
This antibody recognizes human IKKbeta (87 kD). No cross-reactivity with IKKalpha or IKKgamma.
图像Name_1 :
qed-bioscience-anti-ikkbeta-antibody-2121-1.jpg
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-anti-ikkbeta-antibody-2121-2.jpg
应用2 :
免疫细胞化学
图像Name_3 :
qed-bioscience-anti-ikkbeta-antibody-2121-3.jpg
应用3 :
IF
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国