抗体

抗胱氨酸-天冬氨酸蛋白酶9(I20)抗体

2418

100微克

455美元

多克隆

domestic rabbit

未共轭

抗胱氨酸-天冬氨酸蛋白酶9(I20)抗体

抗胱氨酸-天冬氨酸蛋白酶9(I20)抗体(2418)

抗体

抗胱氨酸-天冬氨酸蛋白酶9(I120)兔多克隆抗体

胱氨酸-天冬氨酸蛋白酶9(I120)

人类

胱氨酸-天冬氨酸蛋白酶9(I20)

P55211

胱氨酸-天冬氨酸蛋白酶9

CASP-9, EC 3.4.22.62, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6 [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]

CASP9

NP_001220

多克隆

液体

Lot Specific

100微克

455美元

318.5

2418

免疫印迹

Immunoblotting: use at 1ug/ml. Immunocytochemistry: use at 2ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from HeLa cells.

兔

纯化

经免疫亲和层析纯化

Peptide corresponding to aa 299- 318 of human caspase-9 (accession no. P55211).

凋亡

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3, a protease responsible for proteolytic cleavage of many key proteins involved in apoptosis. Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis induced by a wide variety of apoptosis inducers including TNF , TRAIL, anti-CD95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze-thaw cycles.

这抗体识别全长人类胱氨酸-天冬氨酸蛋白酶9(46kDa)

qed-bioscience-anti-caspase-9-i20-antibody-2418-1.gif

免疫印迹

qed-bioscience-anti-caspase-9-i20-antibody-2418-2.gif

免疫印迹

qed-bioscience-anti-caspase-9-i20-antibody-2418-3.gif

免疫印迹

qed-bioscience-anti-caspase-9-i20-antibody-2418-4.jpg

Immunofluorescence Validation of Caspase 9 in K562 Cells . Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with 2418 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

IF

qed-bioscience-anti-caspase-9-i20-antibody-2418-5.gif

Immunofluorescence Validation of Caspase 9 in HeLa Cells . Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with 2418 at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

IF

qed-bioscience-anti-caspase-9-i20-antibody-2418-6.gif

Immunocytochemistry Validation of Caspase 9 in HeLa Cells . Immunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody (2418) at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

免疫细胞化学