抗体

抗IRAK(CT)抗体

2426

100微克

455美元

多克隆

domestic rabbit

未共轭

抗IRAK(CT)抗体

抗IRAK(CT)抗体(2426)

抗体

抗IRAK(CT)兔多克隆抗体

IRAK(CT)

人类

IRAK(CT)

P51617

Cytoplasm , Nucleus , Lipid droplet

Interleukin-1 receptor-associated kinase 1

IRAK-1, EC 2.7。 11.1

IRAK1

NP_001020413

多克隆

液体

Lot Specific

100微克

455美元

318.5

2426

免疫印迹, 免疫沉淀

Immunoblotting: use at 1:1,000-1:2,000 dilution. Immunoprecipitation: use 2-4 ug antibody per sample. Positive control: Whole cell lysate from HeLa cells or THP-1 cells.

兔

纯化

经免疫亲和层析纯化

Peptide corresponding the C- terminus of human IRAK.

神经科学

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-kappaB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFalpha, LPS, oxidative stress, and mitogens. NF-kappaB is associated with IkappaB in cytoplasm. After stimulation, 1kappaB is phosphorylated and dissociates from NF-kappaB. NF-kappaB enters cell nuclei where it activates an array of genes. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) mediates a signaling cascade leading to NF-kappaB activation. IRAK is associated with IL-1 receptor subunits IL-1RI and IL-1RacP and serves as a signaling molecule to mediate IL-1 responses. IRAK also mediates IL-18-induced NF-kappaB activation.

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. {PubMed:11397809, PubMed:12860405, PubMed:14684752, PubMed:15084582, PubMed:15465816, PubMed:15767370, PubMed:17997719, PubMed:20400509}.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze-thaw cycles.

This antibody recognizes full-length human IRAK (80 kD).

qed-bioscience-anti-irak-ct-antibody-2426-1.gif

免疫印迹

qed-bioscience-anti-irak-ct-antibody-2426-2.gif

免疫印迹

qed-bioscience-anti-irak-ct-antibody-2426-3.gif

免疫印迹

qed-bioscience-anti-irak-ct-antibody-2426-4.gif

Species Activity in Mouse and Rat Cell Lines . Loading: 15 ug of lysates per lane. Antibodies: IRAK 2426 (1 µg/mL,), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

免疫印迹

qed-bioscience-anti-irak-ct-antibody-2426-5.jpg

Immunofluorescence Validation of IRAK in Human HeLa Cells . Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa Cells labeling IRAK with 2426 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

IF

qed-bioscience-anti-irak-ct-antibody-2426-6.jpg

Immunocytochemistry Validation of IRAK in Human HeLa Cells . Immunocytochemical analysis of HeLa cells using anti-IRAK antibody (2426) at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

免疫细胞化学

qed-bioscience-anti-irak-ct-antibody-2426-7.gif

Immunoprecipitation and Overexpression Validation in HEK293T Cells(Schauvliege et al., 2006) . Co-expression of Pellino proteins and IRAK-1 leads to Pellino phosphorylation and IRAK-1 polyubiquitination. (A) E-tagged Pellino proteins were co-expressed with IRAK-1WT and HA–ubiquitin in HEK293T cells. For assessment of IRAK-1 polyubiquitination, the same cellextracts, untreated or treated with phosphatase as described above, were analysed for slower migrating forms of IRAK-1 by Western blotting withanti-IRAK-1 (2426). Ubiquitination was specifically detected by IRAK-1 immunoprecipitation followed by Western blotting with anti-HA antibodies.

免疫印迹

qed-bioscience-anti-irak-ct-antibody-2426-8.gif

KD Validation in Human Chondrocytes (Ahmad et al., 2416) . Chondrocytes were transfected with 250 nM of IRAK1 or control siRNA for 48 h and lysates were analyzed for IRAK1 or β-actin expression levels by immunoblotting. IRAK1 signal was disrupted in IRAK1 KD lysate.

IB