产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗RbAp48抗体
目录 :
3110
规格 :
100微克
价格 :
461美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
11G10
反应物种 :
人类
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 3110 图像 1
图像 2 :
QED Bioscience 3110 图像 2
Immunofluorescence analysis of HeLa, using RbAp48(3110) antibody at 1:100 dilution.
图像 3 :
QED Bioscience 3110 图像 3
SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (3110) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
产品信息
目录号 :
3110
产品名称 :
抗RbAp48抗体
NAME+SKU :
抗RbAp48抗体(3110)
产品_TYPE :
抗体
描述 :
抗RbAp48小鼠单克隆抗体
SPECIFICITY SHORT :
RbAp48
物种活性 :
人类
靶标名 :
RbAp48
UniProt数据库 :
P06400
LOCATION :
Nucleus
蛋白名 :
Retinoblastoma-associated protein
别称 :
p105-Rb, p110-RB1, pRb, Rb, pp110
基因名称 :
视网膜成神经细胞瘤相关蛋白
登录号 :
NP_000312
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
461美元
DISTIRBUTOR价格 :
322.7
SKU号规格1 :
3110
应用 :
免疫印迹, 免疫沉淀, IF
宿主 :
小鼠
格式 :
纯化
抗体亚型 :
IgG2b
克隆编号 :
11G10
免疫原 :
GST fusion protein corresponding to aa 1-425 of human RbAp48.
研究领域 :
Cancer Research
配方 :
PBS, pH值7.4
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
FUNCTION :
Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}.; (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.
STORAGE空调 :
These antibodies are stable for at least protein (p110RB) produced in E. coli. o o Host Species: Mouse Antibody Class: 3101-3106 = IgG1; 3107 = IgG2a. Stabilizers: None Preservatives: None. Available on request.
特异性 :
这抗体识别人类和小鼠RbAp48
图像Name_1 :
qed-bioscience-anti-rbap48-antibody-3110-1.jpg
应用1 :
免疫组化
图像Name_2 :
qed-bioscience-anti-rbap48-antibody-3110-2.jpg
应用2 :
IF
图像Name_3 :
qed-bioscience-anti-rbap48-antibody-3110-3.jpg
应用3 :
免疫印迹
图像Name_4 :
qed-bioscience-anti-rbap48-antibody-3110-4.jpg
图像Caption_4 :
HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [11G10] (3110) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
应用4 :
免疫印迹
图像Name_5 :
qed-bioscience-anti-rbap48-antibody-3110-5.jpg
图像Caption_5 :
RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 ug preimmune mouse IgG B. 5 ug of RbAp48 antibody (3110) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
应用5 :
免疫沉淀
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国