产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗雌激素受体α抗体
目录 :
3335
规格 :
100微克
价格 :
461美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
1F3
反应物种 :
人类
更多信息或购买 :
图像
图像 1 :
Whole cell extract (60 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (3335) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
图像 2 :
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (3335) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
图像 3 :
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (3335) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
产品信息
目录号 :
3335
产品名称 :
抗雌激素受体α抗体
NAME+SKU :
抗雌激素受体α抗体(3335)
产品_TYPE :
抗体
描述 :
抗雌激素受体α小鼠单克隆抗体
SPECIFICITY SHORT :
雌激素受体α
物种活性 :
人类
靶标名 :
雌激素受体α
UniProt数据库 :
P03372
LOCATION :
[Isoform 1]: Nucleus , Cytoplasm , Cell membrane
蛋白名 :
雌激素受体
别称 :
ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
基因名称 :
ESR1
登录号 :
NP_000116
克隆性 :
单克隆
形式 :
液体
浓度 :
1.0毫克/毫升
规格1 :
100微克
标价规格1 :
461美元
DISTIRBUTOR价格 :
322.7
SKU号规格1 :
3335
应用 :
免疫印迹, 免疫沉淀
应用INSTRUCTIONS :
Immunoblotting: use at 1-5ug/ml.
Immunohistochemistry: frozen sections, use at 1-10ug/ml.
FACS: use at 1-10ug/ml.
Immunoprecipitation: use at 1-5ug/ml.
Positive controls: MCF-7 cells and recombinant protein.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
宿主 :
小鼠
格式 :
纯化
纯化 :
Purified by Protein G affinity chromatography.
抗体亚型 :
IgG1
克隆编号 :
1F3
免疫原 :
6-His fusion protein containing the region encoding aa 1-190 of human estrogen receptor-alpha (ER-alpha) expressed in E. coli.
研究领域 :
Cancer Research
配方 :
PBS, pH值7.4, 1毫克/毫升
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
FUNCTION :
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). {PubMed:10681512, PubMed:10816575, PubMed:11477071, PubMed:11682626, PubMed:14764652, PubMed:15078875, PubMed:15891768, PubMed:16043358, PubMed:16617102, PubMed:16684779, PubMed:17922032, PubMed:17932106, PubMed:18247370, PubMed:19350539, PubMed:20074560, PubMed:20705611, PubMed:21330404, PubMed:22083956, PubMed:7651415, PubMed:9328340}.; [Isoform 3]: Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861). {PubMed:10970861, PubMed:21937726}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -70C. Avoid multiple freeze- thaw cycles.
特异性 :
This antibody recognizes human and rabbit ER-alpha, a 65 kD protein and a member of the steroid family of nuclear receptors. ER-alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, ER-alpha can positively or negatively regulate gene transcription.
图像Name_1 :
qed-bioscience-anti-estrogen-receptor-alpha-antibody-3335-1.jpg
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-anti-estrogen-receptor-alpha-antibody-3335-2.jpg
应用2 :
免疫印迹
图像Name_3 :
qed-bioscience-anti-estrogen-receptor-alpha-antibody-3335-3.jpg
应用3 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国