抗体

抗p53抗体

3339

100微克

461美元

单克隆

小鼠

未共轭

D01

抗p53抗体

抗p53抗体(3339)

抗体

抗p53小鼠单克隆抗体

p53

人类

p53

P04637

Cytoplasm , Nucleus , Nucleus, PML body , Endoplasmic reticulum , Mitochondrion matrix , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Cellular tumor antigen p53

Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

TP53

NP_000537

单克隆

液体

Lot Specific

100微克

461美元

322.7

3339

免疫印迹, 免疫沉淀, 免疫组化, 酶联免疫吸附测定

IHC (frozen and paraffin), Immunoblotting. IHC: use at 1-10ug/ml. Antigen retrieval: citrate buffer, pH 6.0, 15 minutes. #3339 on paraffin-embedded human breast carcinoma. #3339 on paraffin-embedded human colon carcinoma. Immunoblotting: use at 1-5ug/ml. A band of 53kDa is detected. #3339 on untreated (-) and treated (+) HCT116 cell extracts. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

小鼠

纯化

IgG2a

D01

重组人类p53

Cancer Research

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). {PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937}.

This antibody is stable for at least one (1) year at -20C or below. Store undiluted product in appropriate aliquots to avoid multiple freeze-thaw cycles.

qed-bioscience-anti-p53-antibody-3339-1.jpg

免疫印迹

qed-bioscience-anti-p53-antibody-3339-2.jpg

免疫印迹

qed-bioscience-anti-p53-antibody-3339-3.jpg

免疫印迹

qed-bioscience-anti-p53-antibody-3339-4.jpg

Untreated (–) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (3339) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

免疫印迹

qed-bioscience-anti-p53-antibody-3339-5.jpg

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. p53 stained by p53 antibody [DO1] (3339) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

免疫组化

qed-bioscience-anti-p53-antibody-3339-6.jpg

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. p53 stained by p53 antibody [DO1] (3339) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

免疫组化

qed-bioscience-anti-p53-antibody-3339-7.jpg

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. p53 stained by p53 antibody [DO1] (3339) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

免疫组化

qed-bioscience-anti-p53-antibody-3339-8.jpg

293T whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (3339) diluted at 1:40000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

免疫印迹