产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗p53抗体
目录 :
3340
规格 :
100微克
价格 :
461美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
240
反应物种 :
人类
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 3340 图像 1
Sample (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 uM cisplatin treatment for 24 hr 10% SDS PAGE 3340 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
图像 2 :
QED Bioscience 3340 图像 2
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53(3340) antibody at 1:200 dilution.
图像 3 :
QED Bioscience 3340 图像 3
Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE 3340 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
产品信息
目录号 :
3340
产品名称 :
抗p53抗体
NAME+SKU :
抗p53抗体(3340)
产品_TYPE :
抗体
描述 :
抗p53小鼠单克隆抗体
SPECIFICITY SHORT :
p53
物种活性 :
人类
靶标名 :
p53
UniProt数据库 :
P04637
LOCATION :
Cytoplasm , Nucleus , Nucleus, PML body , Endoplasmic reticulum , Mitochondrion matrix , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
蛋白名 :
Cellular tumor antigen p53
别称 :
Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
基因名称 :
TP53
登录号 :
NP_000537
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
461美元
DISTIRBUTOR价格 :
322.7
SKU号规格1 :
3340
应用 :
免疫印迹, 免疫沉淀, 免疫组化, 流式细胞仪
应用INSTRUCTIONS :
ELISA, FACS, IHC (frozen and paraffin), Immunoprecipitation, Immunoblotting. IHC: use at 2-4ug/ml. Extensive washing may be necessary. Protein digestion and/or microwave antigen retrieval prior to staining is not required. #3340 on paraffin-embedded HBL435 xenograft. Immunoprecipitation: use at 10ug/mg of lysate. This antibody reacts only with mutant p53 under non-denaturing conditions. Immunoblotting: use at 5-10ug/ml. A band of 53kDa is detected. #3340 on 30ug of 293T (A) and A431 (B) cell lysates. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
宿主 :
小鼠
格式 :
纯化
抗体亚型 :
IgG1
克隆编号 :
240
免疫原 :
凝胶纯化p53含氨基酸14-389人的p53
研究领域 :
Cancer Research
配方 :
PBS, pH值7.4
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
FUNCTION :
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). {PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775, PubMed:15340061, PubMed:17317671, PubMed:17349958, PubMed:19556538, PubMed:20673990, PubMed:20959462, PubMed:22726440, PubMed:24051492, PubMed:9840937}.
STORAGE空调 :
This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.
图像Name_1 :
qed-bioscience-anti-p53-antibody-3340-1.jpg
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-anti-p53-antibody-3340-2.jpg
应用2 :
免疫组化
图像Name_3 :
qed-bioscience-anti-p53-antibody-3340-3.jpg
应用3 :
免疫印迹
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国